Laboratory of Anthropology, Medicine Faculty, University of Granada, Spain.
Forensic Sci Int. 2013 Mar 10;226(1-3):33-7. doi: 10.1016/j.forsciint.2012.11.012. Epub 2012 Dec 31.
The analysis of burned human remains has given rise to many publications in the literature and has caused great interest among forensic specialists and physical anthropologists due to the difficulty in its analysis and interpretation. The main goal of this study has been to measure the changes that occur in bone matrix as a consequence of the increased temperature and establishing categories of histological morphology in relation to fire temperature. To this end, a total of 150 bone cylinders from the ilium obtained by bone biopsy. These samples have been obtained from forensic cadavers and burned at controlled temperatures between 100 and 1100°C in an oven. The samples were fixed in methyl methacrylate and stained with hematoxylin-eosin, Goldner's trichrome and toluidine blue stains. The samples were studied using an optical microscope at 100×. Our study classifies the morphological changes that occur in bone matrix in four stages as a result of the temperature.
对烧焦人类遗骸的分析在文献中引起了广泛关注，引起了法医学家和体质人类学家的极大兴趣，因为其分析和解释具有一定难度。本研究的主要目的是测量骨基质因温度升高而发生的变化，并建立与火灾温度有关的组织形态学分类。为此，共从法医尸体中获得了 150 个取自髂骨的骨圆柱，并在烤箱中以 100 至 1100°C 的受控温度进行燃烧。将这些样本用甲基丙烯酸甲酯固定，并使用苏木精-伊红、戈尔登三色和甲苯胺蓝染色进行染色。使用光学显微镜在 100×下对样本进行研究。我们的研究将由于温度导致的骨基质中发生的形态变化分为四个阶段。
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