Lalande M, Hanauske-Abel H M
Howard Hughes Medical Institute, Division of Genetics, Boston, Massachusetts.
Exp Cell Res. 1990 May;188(1):117-21. doi: 10.1016/0014-4827(90)90285-i.
A novel cell cycle blocking agent profoundly suppressed the proliferation of mitogen-stimulated T lymphocytes. The carboxythiazole derivative arrested cells in the G1 phase of the cell cycle but did not inhibit the induction of cell surface receptors for either interleukin-2 or transferrin. The uncoupling of transferrin receptor expression from DNA synthesis indicated that a previously undefined restriction point in the cell cycle has been identified which occurs after transferrin receptor expression in late G1 and just prior to the initiation of DNA replication in S phase. T cells incubated in an inhibitory dose of the carboxythiazole derivative resumed cell cycle progression subsequent to its removal, indicating that the compound reversibly arrests cells at the late G1 restriction point. In contrast to other techniques which have been inefficient in achieving T cell synchronization, T cells released from the block mediated by the carboxythiazole compound progress through S phase with a considerable degree of synchrony.
一种新型细胞周期阻断剂可显著抑制有丝分裂原刺激的T淋巴细胞的增殖。羧基噻唑衍生物使细胞停滞于细胞周期的G1期，但不抑制白细胞介素-2或转铁蛋白的细胞表面受体的诱导。转铁蛋白受体表达与DNA合成的解偶联表明，已确定细胞周期中一个先前未明确的限制点，该限制点发生在G1晚期转铁蛋白受体表达之后且恰好在S期DNA复制开始之前。在抑制剂量的羧基噻唑衍生物中孵育的T细胞在去除该化合物后恢复细胞周期进程，表明该化合物在G1晚期限制点可逆地阻滞细胞。与在实现T细胞同步化方面效率不高的其他技术相比，从羧基噻唑化合物介导的阻滞中释放的T细胞以相当程度的同步性通过S期。
