通过抑制环磷酸腺苷依赖性蛋白激酶来抑制受精海胆卵中的有丝分裂。

Inhibition of mitosis in fertilized sea urchin eggs by inhibition of the cyclic AMP-dependent protein kinase.

作者信息

Browne C L, Bower W A, Palazzo R E, Rebhun L I

机构信息

Department of Biology, Wake Forest University, Winston-Salem, North Carolina 27109.

出版信息

Exp Cell Res. 1990 May;188(1):122-8. doi: 10.1016/0014-4827(90)90286-j.

DOI:10.1016/0014-4827(90)90286-j

PMID:2328770

Abstract

Inhibition of cAMP-dependent protein kinase activity by microinjection of a specific physiologic protein inhibitor into sea urchin eggs inhibits the first cleavage after fertilization. Inhibition apparently occurs at some time prior to or during formation of the mitotic spindle. Measurement of the total protein kinase activity of sea urchin egg homogenates after fertilization showed that cAMP-dependent phosphorylation increases after fertilization and then declines prior to or at the time of the first cleavage. It is concluded that a cAMP-dependent phosphorylation plays a significant role in events leading to regulation of mitotic spindle assembly.

摘要

通过向海胆卵显微注射一种特定的生理蛋白抑制剂来抑制环磷酸腺苷（cAMP）依赖性蛋白激酶活性，会抑制受精后的第一次卵裂。这种抑制显然发生在有丝分裂纺锤体形成之前或形成过程中的某个时间点。对受精后海胆卵匀浆的总蛋白激酶活性进行测量表明，cAMP依赖性磷酸化在受精后增加，然后在第一次卵裂之前或之时下降。由此得出结论，cAMP依赖性磷酸化在导致有丝分裂纺锤体组装调控的事件中起重要作用。