丝状真菌中一种新型磷脂转移蛋白的纯化与特性分析
Purification and characterization of a novel phospholipid transfer protein from filamentous fungi.
作者信息
Grondin P, Vergnolle C, Chavant L, Kader J C
机构信息
Laboratoire de Cryptogamie, Université Paul Sabatier, Toulouse, France.
出版信息
Int J Biochem. 1990;22(1):93-8. doi: 10.1016/0020-711x(90)90083-f.
PMID:2328823
Abstract
- We have isolated from mycelia of Mucor mucedo, a filamentous fungus, a phospholipid transfer protein. 2. The purification steps were gel filtration, hydroxyapatite chromatography, blue affinity column and fast protein liquid chromatography on anion exchanger. 3. A purified protein was obtained with a molecular mass of 24 kDa and a pI of 5.05 and its N-terminal sequence was established. 4. This protein transfers phosphatidylinositol, as well as phosphatidylcholine and phosphatidylethanolamine.
摘要
- 我们从丝状真菌毛霉的菌丝体中分离出了一种磷脂转移蛋白。2. 纯化步骤包括凝胶过滤、羟基磷灰石色谱、蓝色亲和柱和阴离子交换剂上的快速蛋白质液相色谱。3. 获得了一种分子量为24 kDa、pI为5.05的纯化蛋白，并确定了其N端序列。4. 这种蛋白能转移磷脂酰肌醇，以及磷脂酰胆碱和磷脂酰乙醇胺。
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