Instituto Nacional de Parasitología Mario Fatala Chaben, ANLIS Malbrán, Av. Paseo Colón 568, Buenos Aires, Argentina.
J Assist Reprod Genet. 2013 Mar;30(3):315-23. doi: 10.1007/s10815-012-9914-8. Epub 2013 Jan 4.
Different laboratories around the world have succeeded in establishing human embryonic stem cell (hESC) lines. However, culture conditions vary considerably among the protocols used and the vast majority of the lines at some stage of their creation have been in contact with an animal derived component. One of the main problems to be overcome for the generation of a clinical-grade hESC line is the choice of a substrate and medium that allows derivation and culture, where animal derived components are kept to a minimum or completely excluded.
The following review describes past and more recent achievements in the creation and culturing of hESC. It describes protocols, giving special attention to the matrices and supplements used for derivation, maintainance and cryostorage, considering whether they included defined, undefined and/or animal-derived components in their formulations.
This information shall be useful for the creation and choice of new substrates and supplements for future research in the field of hESC for therapeutic purposes.
世界各地的不同实验室已成功建立人类胚胎干细胞（hESC）系。然而，所用方案之间的培养条件差异很大，而且在创建过程的某个阶段，绝大多数的 hESC 系都与动物源性成分接触过。为了生成临床级 hESC 系，需要克服的主要问题之一是选择一种基质和培养基，使干细胞能够被诱导和培养，同时将动物源性成分的使用减到最少或完全排除。
本综述描述了过去和最近在 hESC 的创建和培养方面的成就。它描述了各种方案，特别关注用于诱导、维持和低温保存的基质和补充剂，考虑它们的配方中是否包含了定义明确、未定义和/或动物源性成分。
这些信息对于未来治疗性 hESC 领域的研究中创建和选择新的基质和补充剂将是有用的。
