Pan Yuanyuan, Huang Haipeng, Meng Jing, Xiao Hongyu, Li Cheng, Meng Lin, Hong Shan, Liu Henan, Wang Xuefeng, Jiang Juquan
Key Laboratory of Soybean Biology of Ministry of Education; Microbiology and Biotechnology, College of Life Sciences, Northeast Agricultural University, Harbin 150030, China.
Wei Sheng Wu Xue Bao. 2012 Oct 4;52(10):1187-94.
To analyze the biodiversity of halotolerant and halophilic bacteria the bacteria in saline-alkaline soils in Songnen Plain, we isolated and purified bacteria samples in the area.
Halotolerant and halophilic bacteria were isolated from the enriched cultures of the saline-alkline soil samples through the traditional culture method, and 16S rRNA sequences were amplified and analyzed for the determination of phylogenetic relationships.
Forty strains were obtained and classified into 34 species, 16 genera, 8 families, 3 phylum (Firmicutes, gamma-Proteobacteria and Actinobacteria) in the Domain Bacteria. The genus Staphylococcus is the predominant group, followed by Halomonas, Oceanbacillus, Bacillus, Kocuria and Pseudomonas. The 16S rRNA sequences of 9 strains showed 97.2 to 99.0% similarities with their closest type strains, suggesting that they may be the potential novel species. The salt tolerance of the strains is mainly concentrated on the 5% - 10% NaCl while alkaline pH resistance of the strains is between pH 9 and 12. Based on the NaCl dependence, 62.5% of the strains were grouped into the halotolerant bacteria, and the rest into the moderate halophiles.
The halotolerant and halophilic bacteria are very rich in the saline-alkaline soils in Songnen Plain, and the genera Staphylococcus and Halomonas are the predominant groups. Almost all the bacteria from this area could tolerate not only at the high concentration of NaCl, but also at high alkaline pH. More importantly, a number of novel species may be included in this area.
为分析松嫩平原盐碱土壤中耐盐和嗜盐细菌的生物多样性，我们对该地区的细菌样本进行了分离和纯化。
通过传统培养方法从盐碱土壤样本的富集培养物中分离出耐盐和嗜盐细菌，并扩增16S rRNA序列，进行系统发育关系分析。
共获得40株菌株，分为34个种、16个属、8个科、细菌域中的3个门（厚壁菌门、γ-变形菌门和放线菌门）。葡萄球菌属是优势菌群，其次是嗜盐单胞菌属、海洋芽孢杆菌属、芽孢杆菌属、考克氏菌属和假单胞菌属。9株菌株的16S rRNA序列与其最相近模式菌株的相似性为97.2%至99.0%，表明它们可能是潜在的新物种。菌株的耐盐性主要集中在5% - 10% NaCl，而耐碱性pH值在pH 9至12之间。根据对NaCl的依赖性，62.5%的菌株被归类为耐盐细菌，其余的为中度嗜盐菌。
松嫩平原盐碱土壤中耐盐和嗜盐细菌非常丰富，葡萄球菌属和嗜盐单胞菌属是优势菌群。该地区几乎所有细菌不仅能耐受高浓度的NaCl，还能耐受高碱性pH值。更重要的是，该地区可能包含一些新物种。
