Xiao L J, Hinman C L
Department of Medicinal and Biological Chemistry, University of Toledo, Ohio.
Int J Immunopharmacol. 1990;12(3):247-54. doi: 10.1016/0192-0561(90)90079-3.
A one-hour preincubation of nonadherent murine spleen cells with a soluble membrane-active cardiotoxin purified from the venom of the Thailand cobra Naja naja siamensis results in the destruction of natural killer (NK) cell activity against YAC-1 target cells in a dose-dependent manner. Prior in vivo induction of interferon production by polyinosinic/polycytidylic acid does not avert the cardiotoxin inhibition of NK function. Loss of complement-mediated lysis of cells capable of binding an NK-1.1 monoclonal antibody suggests that the cardiotoxin directly affects the integrity of the NK cell plasma membrane. Cardiotoxin which has been adsorbed to the surface of polystyrene tissue culture plates retains the ability to lyse splenic T lymphocytes, but loses the ability to interfere with NK activity, as measured either by the release of 51Cr or by the uptake of 3H-thymidine by the target lymphoma cells, suggesting that different parts of the cardiotoxin molecule are responsible for destruction of the two types of lymphocytes.
将从泰国眼镜蛇（眼镜王蛇）毒液中纯化得到的可溶性膜活性心脏毒素与非贴壁小鼠脾细胞进行一小时的预孵育，会导致针对YAC-1靶细胞的自然杀伤（NK）细胞活性以剂量依赖性方式被破坏。预先通过聚肌苷酸/聚胞苷酸在体内诱导干扰素产生并不能避免心脏毒素对NK功能的抑制。能够结合NK-1.1单克隆抗体的细胞的补体介导的细胞溶解作用丧失，这表明心脏毒素直接影响NK细胞质膜的完整性。吸附到聚苯乙烯组织培养板表面的心脏毒素保留了裂解脾T淋巴细胞的能力，但失去了干扰NK活性的能力，这通过靶淋巴瘤细胞释放51Cr或摄取3H-胸腺嘧啶核苷来测量，表明心脏毒素分子的不同部分负责破坏这两种类型的淋巴细胞。
