Department of Biotechnology, Lund University, P.O. Box 124, SE 22 100 Lund, Sweden.
J Chromatogr A. 2013 Jan 25;1274:6-12. doi: 10.1016/j.chroma.2012.10.073. Epub 2012 Dec 7.
In this work, a new macroporous molecularly imprinted cryogel (MIP composite cryogel) was synthesized by glutaraldehyde cross-linking reaction of poly(vinyl alcohol) (PVA) particles and amino-modified molecularly imprinted core-shell nanoparticles. The MIP core-shell nanoparticles were prepared using propranolol as a template by one-pot precipitation polymerization with sequential monomer addition. The characteristics of the MIP composite cryogel were studied by scanning electron microscopy (SEM) and texture analyzer. The macroporous structure of the composite (with the pore size varying from a few micrometers to 100 μm) enabled high mass transfer of particulate-containing fluids. In a solid phase extraction (SPE) process, the efficiency and selectivity of the MIP composite cryogel were investigated, where the cryogel was used as an affinity matrix to remove propranolol from aqueous solution as well as from complex plasma sample without prior protein precipitation. The MIP composite cryogel maintained high selectivity and stability and could be used repeatedly after regeneration.
在这项工作中，通过戊二醛交联反应，将聚（聚乙烯醇）（PVA）颗粒和氨基修饰的分子印迹核壳纳米粒子合成了一种新型的大孔分子印迹冷冻凝胶（MIP 复合冷冻凝胶）。MIP 核壳纳米粒子是通过一锅沉淀聚合反应，在单体顺序添加的情况下，以普萘洛尔为模板制备的。通过扫描电子显微镜（SEM）和纹理分析仪研究了 MIP 复合冷冻凝胶的特性。复合的大孔结构（孔径从几微米到 100 微米不等）使含有颗粒的流体具有较高的传质能力。在固相萃取（SPE）过程中，研究了 MIP 复合冷冻凝胶的效率和选择性，其中冷冻凝胶被用作亲和基质，从水溶液和复杂的血浆样品中去除普萘洛尔，而无需预先进行蛋白质沉淀。MIP 复合冷冻凝胶保持了较高的选择性和稳定性，并且可以在再生后重复使用。
