Equine Research Centre, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa.
J Virol Methods. 2013 Apr;189(1):30-5. doi: 10.1016/j.jviromet.2012.12.014. Epub 2013 Jan 3.
Blood samples collected from 503 suspect cases of African horse sickness (AHS) and another 503 from uninfected, unvaccinated South African horses, as well as 98 samples from horses from an AHS free country, were tested with an AHS virus (AHSV) specific duplex real-time reverse transcription quantitative PCR (RT-qPCR) assay and virus isolation (VI). The diagnostic sensitivity and specificity of this AHSV RT-qPCR assay and VI were estimated using a 2-test 2-population Bayesian latent class model which made no assumptions about the true infection status of the tested animals and allowed for the possibility of conditional dependence (correlation) in test results. Median diagnostic sensitivity and specificity of the AHSV RT-qPCR were 97.8% and 99.9%, respectively. Median diagnostic specificity of virus isolation was >99% whereas the estimated diagnostic sensitivity was 44.2%. The AHSV RT-qPCR assay provides for rapid, high-throughput analysis of samples, and is both analytically and diagnostically sensitive and specific. This assay is potentially highly useful for demonstrating freedom or infection of horses with AHSV, thus it is appropriate that its reproducibility be evaluated in other laboratories as a global standard for detection of AHSV.
从 503 例疑似非洲马瘟（AHS）病例和 503 例来自未感染、未接种南非马的血液样本，以及来自无 AHS 国家的 98 匹马的样本中，使用 AHS 病毒（AHSV）特异性双重实时逆转录定量 PCR（RT-qPCR）检测和病毒分离（VI）进行检测。使用 2 测试 2 种群贝叶斯潜在类别模型估计了这种 AHSV RT-qPCR 检测和 VI 的诊断敏感性和特异性，该模型对测试动物的真实感染状态没有任何假设，并允许测试结果存在条件依赖性（相关性）的可能性。AHSV RT-qPCR 的中位诊断敏感性和特异性分别为 97.8%和 99.9%。病毒分离的中位诊断特异性>99%，而估计的诊断敏感性为 44.2%。AHSV RT-qPCR 检测可快速、高通量地分析样本，具有分析和诊断的敏感性和特异性。该检测法对于证明马匹是否感染 AHSV 非常有用，因此，在其他实验室评估其重复性作为检测 AHSV 的全球标准是合适的。
