Department of Life Science, School of Biology, Hunan University, Changsha 410082, P.R. China.
Int J Mol Med. 2013 Feb;31(2):459-66. doi: 10.3892/ijmm.2012.1225. Epub 2012 Dec 27.
The unique differentiation mechanisms of spermatogenesis suggest the existence of cell type- and stage-specific molecules. Herein, a microarray-based approach was used to identify changes in the gene expression profile in mouse GC-1 spg germ cells transfected with spermatogenesis associated gene 12 (SPATA12). One hundred and eighty-two upregulated genes and 104 downregulated genes with fold changes of ≥2 or ≤0.5 (P≤0.05) in expression were identified. Ten genes were selected for validation of the microarray results using quantitative RT-PCR. The real-time quantitative RT-PCR results were consistent with that of the microarray. The gene ontology (GO) terms for the biological functions of the differentially expressed genes induced by SPATA12 included binding activity and immune response. Biological pathway analysis identified several related pathways which are associated with immune responses, cell adhesion and the developmental process. In addition, we observed that SPATA12 may interact with the β-catenin signaling pathway and that SPATA12 may negatively regulate β-catenin signaling during spermatogenesis.
精子发生的独特分化机制表明存在细胞类型和阶段特异性分子。在此，采用基于微阵列的方法鉴定了转染精子发生相关基因 12（SPATA12）的小鼠 GC-1 spg 精母细胞中基因表达谱的变化。鉴定出 182 个上调基因和 104 个下调基因，其表达倍数变化≥2 或≤0.5（P≤0.05）。选择了 10 个基因用于微阵列结果的定量 RT-PCR 验证。实时定量 RT-PCR 结果与微阵列一致。SPATA12 诱导的差异表达基因的基因本体 (GO) 术语的生物学功能包括结合活性和免疫反应。生物途径分析确定了与免疫反应、细胞黏附和发育过程相关的几个相关途径。此外，我们观察到 SPATA12 可能与 β-连环蛋白信号通路相互作用，并且 SPATA12 可能在精子发生过程中负调控 β-连环蛋白信号通路。
