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拟南芥 aux1rcr1 突变改变了 AUXIN RESISTANT1 的靶向，并阻止了根顶端生长素报告基因 DR5:GUS 的表达。

Arabidopsis aux1rcr1 mutation alters AUXIN RESISTANT1 targeting and prevents expression of the auxin reporter DR5:GUS in the root apex.

机构信息

National Key Laboratory of Plant Molecular Genetics and National Center for Plant Gene Research (Shanghai), Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai, China.

出版信息

J Exp Bot. 2013 Feb;64(4):921-33. doi: 10.1093/jxb/ers371. Epub 2013 Jan 4.

DOI:10.1093/jxb/ers371
PMID:23293348
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3580809/
Abstract

Multilevel interactions of the plant hormones ethylene and auxin coordinately and synergistically regulate many aspects of plant growth and development. This study isolated the AUXIN RESISTANT1 (AUX1) allele aux1(rcr1) (RCR1 for REVERSING CTR1-10 ROOT1) that suppressed the root growth inhibition conferred by the constitutive ethylene-response constitutive triple response1-10 (ctr1-10) allele. The aux1(rcr1) mutation resulted from an L126F substitution at loop 2 of the plasma membrane-associated auxin influx carrier protein AUX1. aux1(rcr1) and the T-DNA insertion mutant aux1-T were both defective in auxin transport and many aspects of the auxin response. Unexpectedly, expression of the auxin-response reporter DR5:GUS in the root apex was substantially prevented by the aux1(rcr1) but not the aux1-T mutation, even in the presence of the wild-type AUX1 allele. Following treatment with the synthetic auxin 1-naphthaleneacetic acid (NAA), DR5:GUS expression in aux1(rcr1) and aux1-T occurred mainly in the root apex and mature zone. NAA-induced DR5:GUS expression in the root apex was markedly prevented by ethylene in genotypes with aux1(rcr1) but not in aux1-T genotypes and the wild type. The effect of aux1(rcr1) on DR5:GUS expression seemed to be associated with AUX1-expressing domains. Green fluorescence protein-fused aux1(rcr1) was localized in the cytoplasm and probably not to the plasma membrane, indicating important roles of the Lys(126) residue at loop 2 in AUX1 targeting. The possible effects of aux1(rcr1) on DR5:GUS expression are discussed.

摘要

植物激素乙烯和生长素的多层次相互作用协同调节植物生长和发育的许多方面。本研究分离了 AUXIN RESISTANT1 (AUX1) 等位基因 aux1(rcr1)（REVERSING CTR1-10 ROOT1 的 RCR1），它抑制了组成型乙烯反应组成型三重反应 1-10 (ctr1-10) 等位基因赋予的根生长抑制。aux1(rcr1) 突变是由于质膜相关生长素流入载体蛋白 AUX1 的环 2 中的 L126F 取代。aux1(rcr1) 和 T-DNA 插入突变体 aux1-T 在生长素运输和许多生长素反应方面都有缺陷。出乎意料的是，根顶端的生长素反应报告基因 DR5:GUS 的表达被 aux1(rcr1) 而不是 aux1-T 突变完全阻止，即使存在野生型 AUX1 等位基因也是如此。用合成生长素 1-萘乙酸 (NAA) 处理后，DR5:GUS 在 aux1(rcr1) 和 aux1-T 中的表达主要发生在根顶端和成熟区。乙烯在 aux1(rcr1) 基因型中显著阻止了 NAA 诱导的 DR5:GUS 在根顶端的表达，但在 aux1-T 基因型和野生型中则没有。aux1(rcr1) 对 DR5:GUS 表达的影响似乎与 AUX1 表达域有关。绿色荧光蛋白融合的 aux1(rcr1) 定位于细胞质中，可能不在质膜上，表明 AUX1 靶向中环 2 上 Lys(126) 残基的重要作用。讨论了 aux1(rcr1) 对 DR5:GUS 表达的可能影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2961/3580809/c3271383b993/exbotj_ers371_f0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2961/3580809/8484f87d29d5/exbotj_ers371_f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2961/3580809/c8699bffe6f0/exbotj_ers371_f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2961/3580809/491300f2aabb/exbotj_ers371_f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2961/3580809/f4aa70dbc225/exbotj_ers371_f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2961/3580809/5e724216cf71/exbotj_ers371_f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2961/3580809/f2e902cd36f7/exbotj_ers371_f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2961/3580809/c3271383b993/exbotj_ers371_f0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2961/3580809/8484f87d29d5/exbotj_ers371_f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2961/3580809/c8699bffe6f0/exbotj_ers371_f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2961/3580809/491300f2aabb/exbotj_ers371_f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2961/3580809/f4aa70dbc225/exbotj_ers371_f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2961/3580809/5e724216cf71/exbotj_ers371_f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2961/3580809/f2e902cd36f7/exbotj_ers371_f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2961/3580809/c3271383b993/exbotj_ers371_f0007.jpg

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