Wang Guodong, Qin Weijun, Zheng Jia, Wei Ming, Zhou Xingchun, Wang He, Wen Weihong
Department of Urology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2013 Jan;29(1):82-4, 88.
To investigate the expression levels of enhancer of zeste homolog 2 (EZH2) and human runt-related transcription factor 3 (RUNX3) in renal cell carcinoma (RCC), and analyze their clinical significance and expression correlation.
Expressions of EZH2 and RUNX3 proteins were analyzed by immunohistochemistry in 56 RCC tissues and corresponding adjacent normal renal tissues, respectively. Statistic analysis was conducted using SPSS17.0 software.
Immunohistochemistry revealed that the positive rates of EZH2 and RUNX3 expressions were 67.9% and 37.5% in RCC tissues, respectively, and they were 28.6% and 67.3% in adjacent normal renal tissues. The expressions of EZHZ and RUNX3 between RCC and normal renal tissues were significantly different (P<0.05). High expression of EZH2 and low expression of RUNX3 in RCC were associated with the clinical stage (P<0.05), but not associated with pathological differentiation, age or gender (P>0.05). No statistically significant negative correlation was observed in the expressions of EZH2 and RUNX3 in RCC (P>0.05).
Both EZH2 and RUNX3 may play important roles in the development and progression of RCC, and the detection on EZH2 and RUNX3 expressions will be helpful for early diagnosis and prognosis prediction of RCC.
探讨zeste同源物2增强子（EZH2）和人类 runt相关转录因子3（RUNX3）在肾细胞癌（RCC）中的表达水平，并分析其临床意义及表达相关性。
分别采用免疫组织化学法检测56例RCC组织及相应癌旁正常肾组织中EZH2和RUNX3蛋白的表达。运用SPSS17.0软件进行统计学分析。
免疫组织化学显示，RCC组织中EZH2和RUNX3表达的阳性率分别为67.9%和37.5%，癌旁正常肾组织中分别为28.6%和67.3%。RCC组织与正常肾组织中EZH2和RUNX3的表达差异有统计学意义（P<0.05）。RCC中EZH2高表达和RUNX3低表达与临床分期相关（P<0.05），但与病理分化、年龄或性别无关（P>0.05）。RCC中EZH2和RUNX3的表达无统计学意义的负相关（P>0.05）。
EZH2和RUNX3可能在RCC的发生发展中起重要作用，检测EZH2和RUNX3的表达有助于RCC的早期诊断和预后预测。
