Kumar S N, Stewart G L, Steven W M, Seelig L L
Department of Cellular Biology and Anatomy, Louisiana State University Medical Center, Shreveport 71130.
J Reprod Immunol. 1990 Mar;17(1):69-78. doi: 10.1016/0165-0378(90)90040-d.
We have previously demonstrated the maternal-to-neonatal transfer of immunity to T. spiralis during lactation and have shown that antigen-specific T lymphocytes, when injected into the mother or orally fed to neonates, can mediate this transfer. To further analyze the T cell subsets involved in conferring this protection, T lymphocytes were isolated from the mesenteric lymph nodes of syngeneic donor rats infected 4-6 days earlier with T. spiralis. The T cells were incubated in vitro with either mouse-anti-rat 0X8 or W3/25 monoclonal antibody, "panned" on plates coated with goat-anti-mouse Ig, and the non-adherent T helper or T cytotoxic/suppressor cells harvested. 100 x 10(6) T helper cells were injected i.v. into mothers once in early lactation and again two days prior to challenging their pups (200 T. spiralis larvae) at 2 weeks of age. This resulted in significant passage of immunity from the mothers to their suckling neonates, worm counts being 59% and 73% of control values 3 and 8 days post-challenge (P less than 0.01). Injection of T-cytotoxic/suppressor cells using the same regimen resulted in significant suppression of immunity in challenged pups, who retained worm counts that were 105% and 145% of control values at 3 and 8 days post-challenge. Synergy between recombined panned T-helper and T cytotoxic/suppressor cells without Ly1+2+3+ amplifier cells was tested by recombining non-adherent panned 0X8 and W3/25 cells. This resulted in no significant expressions of immunity in the pups when compared to controls. The presence of transferred maternal T cells within the neonate was evidenced by the fact that neonates (nursing on immune mothers) had significant (P less than 0.01) delayed footpad reactions to a crude T. spiralis antigen preparation, as compared with neonates nursing on non-immune controls.
我们之前已经证明了在哺乳期间母体向新生儿的抗旋毛虫免疫力转移，并表明抗原特异性T淋巴细胞在注入母体或经口喂给新生儿时可介导这种转移。为了进一步分析参与提供这种保护的T细胞亚群，从4-6天前感染旋毛虫的同基因供体大鼠的肠系膜淋巴结中分离出T淋巴细胞。将T细胞与小鼠抗大鼠OX8或W3/25单克隆抗体在体外孵育，在包被有山羊抗小鼠Ig的平板上进行“淘选”，收获非贴壁的T辅助细胞或T细胞毒性/抑制细胞。在哺乳早期，将100×10⁶个T辅助细胞静脉内注射给母体一次，并在其幼崽2周龄时用200条旋毛虫幼虫攻击前2天再次注射。这导致免疫力从母体显著传递给其哺乳的新生儿，攻击后3天和8天虫数分别为对照值的59%和73%（P<0.01）。使用相同方案注射T细胞毒性/抑制细胞导致受攻击幼崽的免疫力显著抑制，在攻击后3天和8天，其虫数分别为对照值的105%和145%。通过重组非贴壁淘选的OX8和W3/25细胞，测试了无Ly1+2+3+放大细胞的重组淘选T辅助细胞和T细胞毒性/抑制细胞之间的协同作用。与对照相比，这导致幼崽中无显著的免疫力表达。与在非免疫对照母鼠上哺乳的新生儿相比，在免疫母鼠上哺乳的新生儿对粗制旋毛虫抗原制剂的足垫反应显著延迟（P<0.01），这证明了新生儿体内存在转移的母体T细胞。
