Gaspar Emanuelle B, Rosetti Andreza S, Lincopan Nilton, De Gaspari Elizabeth
Embrapa Southern Region Animal Husbandry; Bagé, RS Brazil; Institute of Biomedical Sciences; Department of Microbiology; University of São Paulo; São Paulo, SP Brazil.
Hum Vaccin Immunother. 2013 Mar;9(3):572-81. doi: 10.4161/hv.23237. Epub 2013 Jan 7.
Colonization of the nasopharynx by non-pathogenic Neisseria species, including N. lactamica, has been suggested to lead to the acquisition of natural immunity against Neisseria meningitidis in young children. The aim of this study was to identify a model complex of antigens and adjuvant for immunological preparation against N. meningitidis B, based on cross reactivity with N. lactamica outer membrane vesicles (OMV) antigens and the (DDA-BF) adjuvant. Complexes of 25 µg of OMV in 0.1 mM of DDA-BF were colloidally stable, exhibiting a mean diameter and charge optimal for antigen presentation. Immunogenicity tests for these complexes were performed in mice. A single dose of OMV/DDA-BF was sufficient to induce a (DTH) response, while the same result was achieved only after two doses of OMV/alum. In addition, to achieve total IgG levels that are similar to a single immunization with OMV/DDA-BF, it was necessary to give the mice a second dose of OMV/alum. Moreover, the antibodies induced from a single immunization with OMV/DDA-BF had an intermediate avidity, but antibodies with a similar avidity were only induced by OMV/alum after two immunizations. The use of this novel cationic adjuvant for the first time with a N. lactamica OMV preparation revealed good potential for future vaccine design.
包括乳酸奈瑟菌在内的非致病性奈瑟菌属在鼻咽部的定植，被认为可使幼儿获得针对脑膜炎奈瑟菌的天然免疫力。本研究的目的是基于与乳酸奈瑟菌外膜囊泡（OMV）抗原和（DDA-BF）佐剂的交叉反应，确定一种用于制备抗B型脑膜炎奈瑟菌免疫制剂的抗原和佐剂复合模型。25 µg OMV与0.1 mM DDA-BF形成的复合物具有胶体稳定性，其平均直径和电荷最适合抗原呈递。在小鼠中对这些复合物进行了免疫原性测试。单剂量的OMV/DDA-BF足以诱导迟发型超敏反应（DTH），而同样的结果只有在两剂OMV/明矾后才能实现。此外，为了达到与单剂量OMV/DDA-BF免疫相似的总IgG水平，有必要给小鼠注射第二剂OMV/明矾。而且，单剂量OMV/DDA-BF免疫诱导的抗体具有中等亲和力，但只有在两次免疫后，OMV/明矾才能诱导出具有相似亲和力的抗体。首次将这种新型阳离子佐剂与乳酸奈瑟菌OMV制剂联合使用，显示出其在未来疫苗设计中的良好潜力。
