Carta Anna R, Carboni Ezio, Spiga Saturnino
Department of Biomedical Sciences, University of Cagliari, Cagliari, Italy.
Methods Mol Biol. 2013;964:295-308. doi: 10.1007/978-1-62703-251-3_17.
Parkinson's disease (PD) is characterized by a progressive degeneration of dopamine (DA) neurons and a chronic loss of motor functions. The investigation of progressive degenerative mechanisms and possible neuroprotective approaches for PD depends upon the development of an experimental animal model that reproduces the neuropathology observed in humans. This chapter describes the generation of the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine/probenecid (MPTPp) chronic mouse model of PD. This model displays key features of PD, including impairment of motor and olfactory functions associated with partial loss of tyrosine hydroxylase-positive neurons and DA levels in the brain. The MPTPp mouse model provides an important tool for the study of mechanisms contributing to the pathological dysfunction of PD at the cellular and whole animal level.
帕金森病（PD）的特征是多巴胺（DA）神经元进行性退化以及运动功能的慢性丧失。对PD进行性退化机制和可能的神经保护方法的研究依赖于能够重现人类观察到的神经病理学的实验动物模型的开发。本章描述了1-甲基-4-苯基-1,2,3,6-四氢吡啶/丙磺舒（MPTPp）慢性帕金森病小鼠模型的构建。该模型展现了帕金森病的关键特征，包括与酪氨酸羟化酶阳性神经元部分丧失和脑内多巴胺水平相关的运动和嗅觉功能损害。MPTPp小鼠模型为在细胞和整体动物水平研究导致帕金森病病理功能障碍的机制提供了重要工具。
