Shimasaki Noriko, Campana Dario
Department of Paediatrics, National University of Singapore, Singapore, Singapore.
Methods Mol Biol. 2013;969:203-20. doi: 10.1007/978-1-62703-260-5_13.
Natural killer (NK) cells are emerging as a new tool for cell therapy of cancer. However, some cancer subtypes are relatively resistant to NK cell cytotoxicity. Expression of anti-CD19 chimeric signaling receptors can enhance NK-cell reactivity against CD19+ leukemia and lymphoma cells. Here we describe a method to enforce expression of such receptors in human NK cells relying on electroporation of mRNA and compare it to retroviral transduction of cDNA. These methods are applicable to the reprogramming of NK cells with chimeric receptors specific for other antigens expressed on cancer cells as well as with molecules that can modulate NK cell function.
自然杀伤（NK）细胞正成为癌症细胞治疗的一种新工具。然而，某些癌症亚型对NK细胞的细胞毒性相对耐药。抗CD19嵌合信号受体的表达可增强NK细胞对CD19+白血病和淋巴瘤细胞的反应性。在此，我们描述了一种依靠mRNA电穿孔在人NK细胞中强制表达此类受体的方法，并将其与cDNA的逆转录病毒转导进行比较。这些方法适用于用对癌细胞上表达的其他抗原具有特异性的嵌合受体以及可调节NK细胞功能的分子对NK细胞进行重编程。
