Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.
Leuk Lymphoma. 2013 Aug;54(8):1769-79. doi: 10.3109/10428194.2013.764418. Epub 2013 Feb 25.
Chronic lymphocytic leukemia (CLL) B-cells resemble self-renewing CD5 + B-cells carrying auto/xeno-antigen-reactive B-cell receptors (BCRs) and multiple innate pattern-recognition receptors, such as Toll-like receptors and scavenger receptors. Integration of signals from BCRs with multiple surface membrane receptors determines whether the cells will be proliferating, anergic or apoptotic. To better understand the role of antigen in leukemogenesis, CLL cell lines producing monoclonal antibodies (mAbs) will facilitate structural analysis of antigens and supply DNA for genetic studies. We present here a comprehensive genotypic and phenotypic characterization of available CLL and normal B-cell-derived lymphoblastoid cell lines (LCLs) from the same individuals (n = 17). Authenticity and verification studies of CLL-patient origin were done by IGHV sequencing, fluorescence in situ hybridization (FISH) and DNA/short tandem repeat (STR) fingerprinting. Innate B-cell features, i.e. natural Ab production and CD5 receptors, were present in most CLL cell lines, but in none of the normal LCLs. This panel of immortalized CLL-derived cell lines is a valuable reference representing a renewable source of authentic Abs and DNA.
慢性淋巴细胞白血病 (CLL) B 细胞类似于自我更新的 CD5+携带自身/异抗原反应性 B 细胞受体 (BCR) 的 B 细胞，并且具有多种先天模式识别受体，例如 Toll 样受体和清道夫受体。BCR 与多种细胞膜受体的信号整合决定了细胞是增殖、无能还是凋亡。为了更好地了解抗原在白血病发生中的作用，产生单克隆抗体 (mAb) 的 CLL 细胞系将有助于抗原的结构分析，并提供用于遗传研究的 DNA。我们在此介绍了来自同一个体的可用 CLL 和正常 B 细胞衍生的淋巴母细胞系 (LCL) 的全面基因和表型特征 (n=17)。通过 IGHV 测序、荧光原位杂交 (FISH) 和 DNA/短串联重复 (STR) 指纹分析对 CLL 患者来源进行了真实性和验证研究。大多数 CLL 细胞系都存在先天 B 细胞特征，即天然 Ab 产生和 CD5 受体，但在正常 LCL 中均不存在。该 panel 永生化的 CLL 衍生细胞系是一个有价值的参考，代表了可再生的真实 Abs 和 DNA 来源。
