采用赛沛Xpert EV检测法快速诊断肠道病毒性脑膜炎：一家三级医疗中心的经验及技术评估
Implementation of the Cepheid Xpert EV assay for rapid detection of enteroviral meningitis: experience of a tertiary care center and a technical review.
作者信息
Slika Silva, Abbas Fatmeh, Mahfouz Rami
机构信息
Department of Pathology & Laboratory Medicine, American University of Beirut Medical Center (AUBMC), Beirut, Lebanon.
出版信息
Genet Test Mol Biomarkers. 2013 Mar;17(3):232-5. doi: 10.1089/gtmb.2012.0291. Epub 2013 Jan 8.
AIMS
Rapid molecular detection of enterovirus in cerebrospinal fluid (CSF) has revolutionized the diagnosis and treatment modalities of patients with meningitis and largely affected cost of unnecessary antibiotic use and length of stay in hospitals.
MATERIALS AND METHODS
Between January 2010 and July 2012, we tested 220 CSF samples on the GeneXpert DX real-time polymerase chain reaction system (by Cepheid) using the Xpert EV kit assays. In addition, we reported 14 sample results cross-validated with an external referral laboratory as well as the results of external proficiency testing.
RESULTS
Out of all tested samples, 19% were positive for enterovirus. Furthermore, all referred and proficiency testing specimens were concordant with the peer-reviewed and tested samples.
CONCLUSION
Implementation of the Cepheid Xpert EV assay for rapid detection of enteroviral meningitis has been successful in our laboratory. This molecular diagnostic test is currently utilized by our staff physicians as a major tool in assessment and management of patients with meningitis.
目的
脑脊液（CSF）中肠道病毒的快速分子检测彻底改变了脑膜炎患者的诊断和治疗方式，并在很大程度上影响了不必要抗生素使用的成本以及住院时间。
材料与方法
2010年1月至2012年7月期间，我们使用Xpert EV试剂盒检测，在GeneXpert DX实时聚合酶链反应系统（由Cepheid公司生产）上对220份脑脊液样本进行了检测。此外，我们报告了14份经外部转诊实验室交叉验证的样本结果以及外部能力验证测试的结果。
结果
在所有检测样本中，19%的样本肠道病毒呈阳性。此外，所有转诊样本和能力验证测试样本与同行评审和检测的样本结果一致。
结论
在我们实验室，使用Cepheid Xpert EV检测法快速检测肠道病毒性脑膜炎已取得成功。这种分子诊断测试目前被我们的内科医生用作评估和管理脑膜炎患者的主要工具。
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