Department of Chemical and Biomolecular Engineering, National University of Singapore, Singapore 117576, Singapore.
Bioresour Technol. 2013 Oct;145:233-9. doi: 10.1016/j.biortech.2012.12.053. Epub 2012 Dec 19.
Green and efficient production of biodiesel (FAME) from waste grease containing high amount of free fatty acid (FFA) was achieved by using novel magnetic nanobiocatalyst aggregates (MNA). Thermomyces lanuginosus Lipase (TLL) and Candida antarctica Lipase B (CALB) were covalently immobilized on core-shell structured iron oxide magnetic nanoparticle (80 nm), respectively, followed by freeze-dry to give MNA (13-17 μm) with high yield (80-89%) and high enzyme loading (61 mg TLL or 22 mg CALB per gram MNA). MNA TL showed the best performance among immobilized enzymes known thus for the production of FAME from grease (17 wt.% FFA) with methanol, giving 99% yield in 12 h (3.3 wt.% catalyst). MNA TL was easily separated under magnetic field and reused, retaining 88% productivity in 11th cycle. MNA CA converted >97% FFA in grease (17 wt.% FFA) to FAME in 12 h (0.45 wt.% catalyst), being useful in two-step transformation of grease to biodiesel.
通过使用新型磁性纳米生物催化剂聚集体（MNA），从含有高游离脂肪酸（FFA）的废油脂中绿色高效地生产生物柴油（FAME）。分别将嗜热真菌脂肪酶（TLL）和南极假丝酵母脂肪酶 B（CALB）共价固定在核壳结构的氧化铁磁性纳米颗粒（80nm）上，然后进行冷冻干燥，得到产率高（80-89%）和酶载量大（每克 MNA 61mg TLL 或 22mg CALB）的 MNA（13-17μm）。在已知用于从油脂（17wt.%FFA）生产 FAME 的固定化酶中，MNA TL 表现出最佳性能，在 12 小时内甲醇转化率为 99%（3.3wt.%催化剂）。MNA TL 可在磁场下轻松分离并重复使用，在第 11 次循环中保留 88%的生产力。MNA CA 在 12 小时内将油脂（17wt.%FFA）中的>97%FFA 转化为 FAME（0.45wt.%催化剂），在油脂两步转化为生物柴油中具有应用价值。
