Audenaert Dominique, De Rybel Bert, Nguyen Long, Beeckman Tom
Department of Plant Systems Biology, VIB, Ghent, Belgium.
Methods Mol Biol. 2013;959:189-95. doi: 10.1007/978-1-62703-221-6_12.
Development of the root system is essential for proper plant growth and development. Extension of the root system is achieved by the continuous establishment of new meristems in existing parental root tissues, which leads to the development of lateral roots. This process of lateral root organogenesis consists of different developmental stages, which are all controlled by the plant hormone auxin. In this chapter, we describe a screening method in Arabidopsis thaliana to identify small synthetic molecules that interfere with the process of lateral root development during specific developmental stages.
根系发育对于植物的正常生长和发育至关重要。根系的延伸是通过在现有的亲本根组织中不断建立新的分生组织来实现的，这会导致侧根的发育。侧根器官发生的这个过程由不同的发育阶段组成，这些阶段均受植物激素生长素的控制。在本章中，我们描述了一种在拟南芥中进行筛选的方法，以鉴定在特定发育阶段干扰侧根发育过程的小分子合成化合物。
