Department of Biotechnology Engineering, Ambala College of Engineering and Applied Research, Ambala, India.
Prep Biochem Biotechnol. 2013;43(2):152-62. doi: 10.1080/10826068.2012.712589.
Hydrolytic enzymes released by the microorganisms in activated sludge are responsible for the organic matter degradation there; however, the optimal extraction procedure of this valuable resource has not been well established until now. In this study protease and lipase were extracted from activated sludge using ultrasound disintegration combined with a nonionic detergent (Triton X-100) and cation-exchange resin (CER) in combination for the extraction of protease and lipase. It was observed that the concentration of 0.1% and 1% Triton X-100 has a strong influence for the extraction of lipase and protease respectively. Closer study of the enzyme extraction process is essential for different enzymes from activated sludge process.
水解酶由活性污泥中的微生物释放，负责那里的有机物降解；然而，到目前为止，这种有价值资源的最佳提取程序还没有很好地建立起来。在这项研究中，蛋白酶和脂肪酶从活性污泥中提取出来，使用超声分解与非离子洗涤剂（Triton X-100）和阳离子交换树脂（CER）相结合，分别提取蛋白酶和脂肪酶。结果表明，0.1%和1%Triton X-100 的浓度对脂肪酶和蛋白酶的提取有很强的影响。对活性污泥工艺中不同酶的酶提取过程进行更深入的研究是必要的。
