Chen Xue, Zhou Zhen, Liu Feng, Zhang Pei, Guo Jian-xin, Wang Wei
Department of Ophthalmology, Peking University Third Hospital, Beijing, China.
Zhonghua Yan Ke Za Zhi. 2012 Oct;48(10):915-9.
To explore the depth of invasion of limbal tumors using in vivo confocal microscope and to compare the result to that in histopathological findings.
Five cases of limbal tumors with clinical diagnosis of squamous cell carcinoma were evaluated with in vivo confocal microscope. Tumor excision and lamella corneal transplantation were performed for each patient. The results of confocal microscopy were compared to that in histopathologic sections.
One case of invasive squamous carcinoma, three cases of intraepithelial dysphasia and one case of squamous papilloma were identified by histopathological examination. The main pathological features could be recognized by confocal microscope images, including papillomatous hyperplasia, cytonuclear atypias, and activation of dendritic cells. Intersection of neoplasm and normal tissue could be displayed by oblique scanning.
With appropriated oblique scanning, in vivo confocal microscopy can provide information of cellular structure and the depth of invasion, this is useful in the diagnosis and management of limbal neoplasm. However, the identification of tumor cell phenotypes still needs ex vivo histopathological examination.
利用活体共聚焦显微镜探讨角膜缘肿瘤的浸润深度，并将结果与组织病理学检查结果进行比较。
对5例临床诊断为鳞状细胞癌的角膜缘肿瘤患者进行活体共聚焦显微镜评估。对每位患者进行肿瘤切除和板层角膜移植。将共聚焦显微镜检查结果与组织病理学切片结果进行比较。
组织病理学检查发现1例浸润性鳞状癌、3例上皮内发育异常和1例鳞状乳头状瘤。共聚焦显微镜图像可识别主要病理特征，包括乳头状增生、细胞核异型性和树突状细胞活化。通过斜向扫描可显示肿瘤与正常组织的交界处。
通过适当的斜向扫描，活体共聚焦显微镜可提供细胞结构和浸润深度信息，这对角膜缘肿瘤的诊断和治疗有用。然而，肿瘤细胞表型的鉴定仍需离体组织病理学检查。
