Department of Biomedical Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA.
Artif Organs. 2013 Jan;37(1):E9-E17. doi: 10.1111/aor.12021.
This work demonstrates the use of a continuous online monitoring system for tracking systemic inflammation biomarkers during cardiopulmonary bypass (CPB) procedures. The ability to monitor inflammation biomarkers during CPB will allow surgical teams to actively treat inflammation and reduce harmful effects on postoperative morbidity and mortality, enabling improved patient outcomes. A microfluidic device has been designed which allows automation of the individual processing steps of a microbead immunoassay to allow continuous tracking of antigen concentrations. Preliminary experiments have demonstrated that the results produced by the microimmunoassay are comparable to results produced from a standard enzyme-linked immunosorbent assay (r = 0.98). Additionally, integration of the assay with a simulated CPB circuit has been demonstrated with temporal tracking of C3a concentrations within blood continuously sampled from the circuit. The presented work describes the motivation, design challenges, and preliminary experimental results of this project.
这项工作展示了一种连续在线监测系统在体外循环 (CPB) 过程中跟踪系统性炎症生物标志物的应用。在 CPB 过程中监测炎症生物标志物的能力将使外科团队能够积极治疗炎症，降低对术后发病率和死亡率的有害影响，从而改善患者的预后。已经设计了一种微流控装置，该装置允许微珠免疫测定的各个处理步骤自动化，从而能够连续跟踪抗原浓度。初步实验表明，微免疫测定产生的结果与标准酶联免疫吸附测定 (r = 0.98) 产生的结果相当。此外，还通过连续从模拟 CPB 回路中采样的血液中对 C3a 浓度进行时间跟踪，证明了该测定与模拟 CPB 回路的集成。本文介绍了该项目的动机、设计挑战和初步实验结果。
