Departamento de Gerontologia, Universidade Federal de São Carlos, São Carlos, Brazil.
Dement Geriatr Cogn Disord. 2013;35(1-2):58-66. doi: 10.1159/000345983. Epub 2013 Jan 9.
Alzheimer's disease (AD) is the most common cause of dementia in people above age 65. Platelet studies with ADAM10 have shown that its expression is reduced in AD patients. The aim of this research was to compare the platelet levels of ADAM10 protein in two Brazilian elderly groups, considering the stages of the disease. The SDS-PAGE technique followed by Western blotting was used. Data were analyzed using comparison, correlation and association statistical methods. The results showed reduced platelet ADAM10 levels in AD elderly compared to non-AD subjects. The disease progression intensified this reduction. ADAM10 was the only statistically significant variable (p = 0.01) to increase the AD occurrence probability. The cutoff value of 0.4212 in the receiver operating characteristic curve captured sensitivity and specificity of 70 and 80.77%, respectively. Together with other clinical criteria, ADAM10 seems to be a relevant biomarker tool for early and accurate AD diagnosis.
阿尔茨海默病（AD）是 65 岁以上人群中最常见的痴呆症病因。对 ADAM10 的血小板研究表明，其在 AD 患者中的表达降低。本研究的目的是比较两个巴西老年人群的 ADAM10 蛋白血小板水平，同时考虑疾病的阶段。使用 SDS-PAGE 技术结合 Western blot 进行分析。使用比较、相关性和关联统计方法分析数据。结果表明，AD 老年患者的血小板 ADAM10 水平低于非 AD 受试者。疾病的进展加剧了这种减少。ADAM10 是唯一具有统计学意义的变量（p = 0.01），可以增加 AD 发生的概率。在受试者工作特征曲线中，截断值为 0.4212，其灵敏度和特异性分别为 70%和 80.77%。与其他临床标准一起，ADAM10 似乎是一种用于早期和准确 AD 诊断的相关生物标志物工具。
