Departamento de Gerontologia, UFSCar, São Carlos, São Paulo, Brazil.
Int J Geriatr Psychiatry. 2014 Apr;29(4):414-20. doi: 10.1002/gps.4020. Epub 2013 Aug 22.
Earlier studies have demonstrated that a disintegrin and metallopeptidase 10 (ADAM10) levels are reduced in Alzheimer's disease (AD) patients compared with healthy subjects. The objective of this study was to evaluate whether platelet ADAM10 levels correlates with the clock drawing test (CDT) scores, which is a simple and a reliable measure of visuospatial ability and executive function in AD patients.
Thirty elderly patients with probable AD and 25 healthy patients forming the control group, matched by age, gender, and educational level, were evaluated. Platelet proteins were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis, and ADAM10 was identified by western blotting. The Spearman's correlation coefficient between ADAM10 and CDT was obtained for each group. The areas under the curves were used to compare the receiver operating characteristic curves.
The CDT scores and platelet ADAM10 expression were significantly different between patients with AD and controls and also along the disease's progression. In AD patients, there was a positive correlation between ADAM10 expression and CDT scores. Among non-AD subjects, no correlation was found. The combination of ADAM10 and CDT was significantly better to confirm the AD diagnosis than the AUCs of ADAM10 and CDT separately.
The association of blood-based biomarkers, such as ADAM10, and cognitive tests may be helpful for a more reliable AD diagnosis.
早期研究表明，与健康受试者相比，阿尔茨海默病（AD）患者的解整合素金属蛋白酶 10（ADAM10）水平降低。本研究的目的是评估血小板 ADAM10 水平是否与画钟测验（CDT）评分相关，CDT 是评估 AD 患者视空间能力和执行功能的一种简单可靠的方法。
对 30 名疑似 AD 的老年患者和 25 名年龄、性别和教育程度相匹配的健康患者（对照组）进行评估。通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳分析血小板蛋白，并通过 Western blot 鉴定 ADAM10。对每组 ADAM10 与 CDT 之间的 Spearman 相关系数进行了分析。比较了受试者工作特征曲线下的面积。
AD 患者与对照组之间的 CDT 评分和血小板 ADAM10 表达存在显著差异，且随疾病进展而变化。在 AD 患者中，ADAM10 表达与 CDT 评分呈正相关。在非 AD 患者中，未发现相关性。与 ADAM10 和 CDT 的 AUC 相比，ADAM10 和 CDT 的组合能更显著地确认 AD 诊断。
血液生物标志物（如 ADAM10）与认知测试的关联可能有助于更可靠地诊断 AD。
