Nakamura M, Itano T, Yamaguchi F, Mizobuchi M, Tokuda M, Matsui H, Etoh S, Hosokawa K, Ohmoto T, Hatase O
Department of Neuropsychiatry, Kagawa Medical School, Japan.
Acta Med Okayama. 1990 Feb;44(1):1-8. doi: 10.18926/AMO/30465.
Peptides and proteins in the extracellular space in the central nervous system were investigated in vivo using an intracerebral microdialysis probe. The molecular cut-off of the hollow fiber which was used for the probe was approximately 100 kDa. We examined recovery rates of several compounds in vitro. The recovery rates of proteins and peptides were between 7-28%, with the exceptions of substance P and insulin-like growth factor I. The recovery rates of monoamines and their metabolites were 22-40%. In in vivo studies, two major proteins with apparent molecular weights of 62 kDa and 12 kDa, and several minor proteins (28 kDa, 43 kDa, 52 kDa and 70 kDa) were detected by SDS-polyacrylamide gel electrophoresis in the dialysate from a probe implanted in the striatum of anesthetized rats. These results suggest that the newly developed, intracerebral microdialysis probe might be useful for investigating the dynamic changes of peptides and proteins in the central nervous system.
使用脑内微透析探针在体内研究中枢神经系统细胞外空间中的肽和蛋白质。用于该探针的中空纤维的分子截留量约为100 kDa。我们在体外检测了几种化合物的回收率。蛋白质和肽的回收率在7%-28%之间，P物质和胰岛素样生长因子I除外。单胺及其代谢物的回收率为22%-40%。在体内研究中，通过SDS-聚丙烯酰胺凝胶电泳在植入麻醉大鼠纹状体的探针的透析液中检测到两种表观分子量分别为62 kDa和12 kDa的主要蛋白质，以及几种次要蛋白质（28 kDa、43 kDa、52 kDa和70 kDa）。这些结果表明，新开发的脑内微透析探针可能有助于研究中枢神经系统中肽和蛋白质的动态变化。
