Liang Hong-Wei, Li Zhong, Zou Gui-Wei
Yangtze River Fisheries Research Institute, The Chinese Academy of Fisheries Sciences, Wuhan 430223, PR China.
Mitochondrial DNA. 2013 Jun;24(3):222-4. doi: 10.3109/19401736.2012.752471. Epub 2013 Jan 11.
The polymerase chain reaction method was employed to obtain the complete mitochondrial genome of Leiocassis crassilabris from the Yangtze River, China. The mitogenome of L. crassilabris is 16,534 bp in length, consisting of 37 genes coding for 13 proteins, 22 transfer RNAs, 2 ribosomal RNAs, and 1 control region. Its gene arrangement pattern was identical with that of most vertebrates. Overall base composition of mitogenome is estimated to be 31.8% A, 26.9% T, 26.4% C, and 14.9% G, with a high A+T content (58.7%). This is the first report on the complete mitogenome sequence of L. crassilabris, which can provide a basic data for the studies on phylogenetic analysis and conservation genetics.
采用聚合酶链反应方法，从中国长江的厚唇裸重唇鱼中获得了完整的线粒体基因组。厚唇裸重唇鱼的线粒体基因组长度为16534 bp，由37个基因组成，编码13种蛋白质、22种转移RNA、2种核糖体RNA和1个控制区。其基因排列模式与大多数脊椎动物相同。线粒体基因组的总体碱基组成为：A占31.8%、T占26.9%、C占26.4%、G占14.9%，A+T含量较高（58.7%）。这是关于厚唇裸重唇鱼完整线粒体基因组序列的首次报道，可为系统发育分析和保护遗传学研究提供基础数据。
