蛋黄中三氯杀螨醇及其代谢物的色谱测定

Chromatographic determination of dicofol and metabolites in egg yolks.

作者信息

Mourer C R, Hall G L, Whitehead W E, Shibamoto T, Shull L R, Schwarzbach S E

机构信息

Department of Environmental Toxicology, University of California, Davis 95616.

出版信息

Arch Environ Contam Toxicol. 1990 Jan-Feb;19(1):154-6. doi: 10.1007/BF01059825.

DOI:10.1007/BF01059825

PMID:2331150

Abstract

Egg yolk was spiked with p,p'-dicofol (p,p'-DCF) (0.1-2.0 micrograms/gm), p,p'-dichlorobenzophenone (p,p'-DCBP) (0.1-2.0 micrograms/gm), and 1,1-bis(4-chlorophenyl)-2,2-dichloroethylene (p,p'-DDE) (0.05-1.0 micrograms/gm). The fortified egg yolk (2-5 g) was mixed with acetonitrile to extract non-fat organic materials. After removal of acetonitrile, the spiked chemicals were separated with a column chromatograph packed with acid alumina. Recovery efficiencies for p,p'-DCBP and p,p'-DDE were determined by gas chromatography, and for p,p'-dicofol by high performance liquid chromatography. The recovery efficiencies for p,p'-dicofol, p,p'-DCBP and p,p'-DDE were 77.2-93.8%, 84.1-101.1%, and 88.5-96.0%, respectively.

摘要

在蛋黄中添加了p,p'-三氯杀螨醇（p,p'-DCF）（0.1 - 2.0微克/克）、p,p'-二氯二苯甲酮（p,p'-DCBP）（0.1 - 2.0微克/克）和1,1-双（4-氯苯基）-2,2-二氯乙烯（p,p'-DDE）（0.05 - 1.0微克/克）。将强化后的蛋黄（2 - 5克）与乙腈混合以提取非脂肪有机物质。去除乙腈后，用装有酸性氧化铝的柱色谱分离添加的化学物质。通过气相色谱法测定p,p'-DCBP和p,p'-DDE的回收率，通过高效液相色谱法测定p,p'-三氯杀螨醇的回收率。p,p'-三氯杀螨醇、p,p'-DCBP和p,p'-DDE的回收率分别为77.2 - 93.8%、84.1 - 101.1%和88.5 - 96.0%。