口腔颗粒细胞瘤中染色体 9p 和 17p 杂合性丢失的证据：一项初步研究。
Evidence for loss of heterozygosity (LOH) at chromosomes 9p and 17p in oral granular cell tumors: a pilot study.
机构信息
Department of Pathology, Biological Sciences Institute, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
出版信息
Oral Surg Oral Med Oral Pathol Oral Radiol. 2013 Feb;115(2):249-53. doi: 10.1016/j.oooo.2012.11.011.
OBJECTIVE
The objective of this study was to assess loss of heterozygosity (LOH) in tumor suppressor gene loci in oral granular cell tumors (GCTs).
STUDY DESIGN
We assessed LOH in 8 samples of oral GCT using polymorphic microsatellite markers at chromosome regions 3p, 9p, 11q, and 17p, flanking areas close to tumor suppressor genes. We further performed immunohistochemistry to detect the p53 and Ki-67 proteins and associated these expressions with the molecular results.
RESULTS
Five samples showed LOH in 3 markers at chromosomes 9p and 17p (markers P53, AFM238WF2 and D9S162) with fraction of allelic loss of 42.8% for each of these markers. No LOH was identified in any other chromosome. LOH was not associated with the immunohistochemical expression of p53 and Ki-67.
CONCLUSIONS
The present study shows LOH at chromosomes 9p and 17p in oral GCTs.
目的
本研究旨在评估口腔颗粒细胞瘤（GCT）中肿瘤抑制基因座杂合性丢失（LOH）。
研究设计
我们使用染色体 3p、9p、11q 和 17p 区域附近的多态性微卫星标记物评估了 8 例口腔 GCT 中的 LOH，这些区域靠近肿瘤抑制基因。我们进一步进行了免疫组织化学检测，以检测 p53 和 Ki-67 蛋白，并将这些表达与分子结果相关联。
结果
5 个样本在染色体 9p 和 17p 上的 3 个标记物（标记物 P53、AFM238WF2 和 D9S162）中显示 LOH，每个标记物的等位基因丢失率为 42.8%。在其他染色体上未发现 LOH。LOH 与 p53 和 Ki-67 的免疫组织化学表达无关。
结论
本研究显示口腔 GCT 中存在染色体 9p 和 17p 的 LOH。
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