Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Facultad de Química, Bioquímica y Farmacia, UNSL, San Luis, Argentina.
J Steroid Biochem Mol Biol. 2013 May;135:60-6. doi: 10.1016/j.jsbmb.2012.12.018. Epub 2013 Jan 8.
The aims of this work were to investigate if oestradiol 10(-8)M in the incubation media of either the ovary alone (OV) or the ganglion compartment of an ex vivo coeliac ganglion-superior ovarian nerve-ovary system (a) modifies the release of ovarian progesterone (P4) and oestradiol (E2) on dioestrus II, and (b) modifies the ovarian gene expression of 3β-HSD and 20α-HSD enzymes and markers of apoptosis. The concentration of ovarian P4 release was measured in both experimental schemes, and ovarian P4 and E2 in the ex vivo system by RIA at different times. The expression of 3β-hydroxysteroid dehydrogenase, 20α-hydroxysteroid dehydrogenase and antiapoptotic bcl-2 and proapoptotic bax by RT-PCR were determined. E2 added in the coeliac ganglion caused an increase in the ovarian release of the P4, E2 and 3β-HSD, while in the ovary incubation alone it decreased P4 and 3β-HSD but increased and 20α-HSD and bax/bcl-2 ratio. It is concluded that through a direct effect on the ovary, E2 promotes luteal regression in DII rats, but the addition of E2 in the coeliac ganglion does not have the same effect. The peripheral nervous system, through the superior ovarian nerve, has a protective effect against the apoptotic mechanism on DII.
这项工作的目的是研究在发情期 II 时，卵巢单独（OV）或腹腔神经节-卵巢神经-卵巢系统（a）的神经节室中培养介质中添加 10(-8)M 的雌二醇是否会：(a) 改变卵巢孕激素 (P4) 和雌二醇 (E2) 的释放；(b) 改变卵巢 3β-HSD 和 20α-HSD 酶以及细胞凋亡标志物的基因表达。在这两种实验方案中都测量了卵巢 P4 释放的浓度，并通过 RIA 在不同时间测量了离体系统中的卵巢 P4 和 E2。通过 RT-PCR 确定了 3β-羟甾脱氢酶、20α-羟甾脱氢酶以及抗凋亡 bcl-2 和促凋亡 bax 的表达。添加到腹腔神经节中的 E2 导致卵巢释放 P4、E2 和 3β-HSD 增加，而单独在卵巢培养中，它会降低 P4 和 3β-HSD，但会增加 20α-HSD 和 bax/bcl-2 比值。研究结论为，通过对卵巢的直接作用，E2 促进了发情期 II 大鼠黄体的退化，但在腹腔神经节中添加 E2 则没有相同的效果。外周神经系统通过卵巢神经对发情期 II 时的细胞凋亡机制具有保护作用。
