de Boer P, Redi C A, Garagna S, Winking H
Department of Genetics, Agricultural University, Wageningen, The Netherlands.
Mol Reprod Dev. 1990 Mar;25(3):297-301. doi: 10.1002/mrd.1080250312.
The level of SH-group oxidation in spermatozoa from the cauda epididymis was measured by a cytofluorometric method in chromosomally normal mice and two chromosome mutants. The first one, a tertiary trisomic karyotype (Ts(1(13]7OH), is characterized by severe oligospermia and high levels (approximately 75%) of malformed spermatozoa. The second, a hybrid between two European feral mouse stocks, is heterozygous for multiple Robertsonian translocations and produces exclusively aneuploid spermatozoa. Neither the severe teratospermiogenesis nor the severe aneuploidy was reflected in total SH-group fluorescence values nor in free SH-group fluorescence. It is concluded that both the production of protamines and protamine cross linking by S-S bridge formation are rather autonomous processes during spermatogenesis because 1) the increased DNA variance of the aneuploid spermatozoa is not reflected in an increased variance of the total and free SH-groups, 2) aneuploidy for the protamine gene carrying chromosome 16 is not reflected by the SH-group values for individual spermatozoa, and 3) protamine production and cross linking are independent of the mild to severe terataspermiogenesis in the tertiary trisomic karyotype.
采用细胞荧光测定法，对染色体正常的小鼠以及两种染色体突变体附睾尾部精子中的SH基团氧化水平进行了测量。第一种突变体是三级三体核型（Ts(1(13]7OH)），其特征为严重少精症以及高水平（约75%）的畸形精子。第二种是两种欧洲野生小鼠品系的杂交种，对多个罗伯逊易位呈杂合状态，只产生非整倍体精子。严重的畸形精子生成和严重的非整倍体现象，在总SH基团荧光值和游离SH基团荧光中均未体现。得出的结论是，在精子发生过程中，鱼精蛋白的产生以及通过二硫键形成进行的鱼精蛋白交联都是相当自主的过程，原因如下：1）非整倍体精子中增加的DNA变异，并未反映在总SH基团和游离SH基团变异的增加上；2）携带鱼精蛋白基因的16号染色体的非整倍体现象，并未通过单个精子的SH基团值体现出来；3）在三级三体核型中，鱼精蛋白的产生和交联与轻度至重度的畸形精子生成无关。
