Yang Wenjiang, Zhao Zuoquan, Fang Wei, Zhang Xianzhong
Key Laboratory of Nuclear Analytical Techniques, Beijing Engineering Research Center of Radiographic Techniques and Equipment, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, China.
Appl Radiat Isot. 2013 Apr;74:1-5. doi: 10.1016/j.apradiso.2012.12.009. Epub 2012 Dec 23.
Diethylenetriaminepentaacetic acid neolactosyl human serum albumin (DTPA-LSA) was prepared and labeled with technetium-99m. The labeling conditions of (99m)Tc-DTPA-LSA were optimized, and lyophilized kit was developed for instant preparing of (99m)Tc-DTPA-LSA. (99m)Tc-DTPA-LSA showed high liver uptake in normal mice (>96% ID/g at 5min after injection), and it could be blocked significantly by pre-injecting free neogalactosylalbumin (NGA). Single photon emission computed tomography (SPECT) study was performed in normal Japanese White rabbits and SPECT images with high quality were obtained at 15, 30, 60, and 120min after injection of the radiotracer. The promising biological properties of (99m)Tc-DTPA-LSA combined with the development of reliable and instant lyophilized DTPA-LSA kit afford the opportunity of hepatic receptor imaging for routine clinical assessment of hepatic function.
制备了二乙烯三胺五乙酸新乳糖基人血清白蛋白（DTPA-LSA）并用99m锝进行标记。优化了（99m）Tc-DTPA-LSA的标记条件，并开发了冻干试剂盒用于即时制备（99m）Tc-DTPA-LSA。（99m）Tc-DTPA-LSA在正常小鼠中显示出高肝脏摄取（注射后5分钟时>96% ID/g），并且预先注射游离新半乳糖基白蛋白（NGA）可显著阻断其摄取。在正常日本白兔中进行了单光子发射计算机断层扫描（SPECT）研究，并在注射放射性示踪剂后15、30、60和120分钟获得了高质量的SPECT图像。（99m）Tc-DTPA-LSA具有良好的生物学特性，结合可靠且即时的冻干DTPA-LSA试剂盒的开发，为肝功能的常规临床评估提供了肝脏受体成像的机会。
