Department of Studies and Research in Industrial Chemistry, School of Chemical Sciences, Kuvempu University, Shankaraghatta 577 451, India.
Spectrochim Acta A Mol Biomol Spectrosc. 2013 Mar 15;105:229-37. doi: 10.1016/j.saa.2012.12.011. Epub 2012 Dec 14.
New metal complexes of the type M(nih)(L)(n)·xH(2)O and M(nih)(2)·xH(2)O (where M=Co(III) or Ni(II), L=1,10-phenanthroline (phen)/or 2,2' bipyridine (bpy), nih=2-hydroxy-1-naphthaldehyde isonicotinoyl hydrazone, n=2 or 1 and x=3 or 2) have been synthesized and characterized by elemental analysis, magnetic, IR and (1)H NMR spectral data. The electronic and magnetic moment 2.97-3.07 B.M. data infers octahedral geometry for all the complexes. The IR data reveals that Schiff base (nih) form coordination bond with the metal ion through azomethine-nitrogen, phenolic-oxygen and carbonyl-oxygen in a tridentate fashion. In addition, DNA-binding properties of these six metal complexes were investigated using absorption spectroscopy, viscosity measurements and thermal denaturation methods. The results indicated that the nickel(II) complex strongly bind with calf-thymus DNA with intrinsic DNA binding constant K(b) value of 4.9×10(4) M(-1) for (3), 4.2×10(4) M(-1) for (4), presumably via an intercalation mechanism compared to cobalt(III) complex with K(b) value of 4.6×10(4) M(-1) (1) and 4.1×10(4) M(-1) (2). The DNA Photoclevage experiment shows that, the complexes act as effective DNA cleavage agent.
新型M(nih)(L)(n)·xH(2)O 和 M(nih)(2)·xH(2)O（其中 M=Co(III) 或 Ni(II)，L=1,10-菲啰啉（phen）/或 2,2'-联吡啶（bpy），nih=2-羟基-1-萘醛异烟酰腙，n=2 或 1，x=3 或 2）金属配合物已经通过元素分析、磁性、IR 和（1）H NMR 光谱数据进行了合成和表征。电子和磁矩 2.97-3.07 B.M.数据推断所有配合物均为八面体几何构型。IR 数据表明席夫碱（nih）通过亚胺氮、酚氧和羰基氧以三齿配位方式与金属离子形成配位键。此外，还通过吸收光谱、粘度测量和热变性方法研究了这些六种金属配合物的 DNA 结合性质。结果表明，镍（II）配合物与小牛胸腺 DNA 具有较强的结合能力，其固有 DNA 结合常数 K(b)值分别为 4.9×10(4) M(-1)（3）、4.2×10(4) M(-1)（4），可能通过嵌入机制与钴（III）配合物相比，K(b)值分别为 4.6×10(4) M(-1)（1）和 4.1×10(4) M(-1)（2）。DNA 光裂解实验表明，这些配合物是有效的 DNA 切割剂。
