Institute of Organic Chemistry, Karlsruhe Institute of Technology, Fritz-Haber-Weg 6, 76131 Karlsruhe, Germany.
Photochem Photobiol Sci. 2013 May;12(5):722-4. doi: 10.1039/c2pp25366f.
The insertion of cyanine dye azides as energy donor dyes via postsynthetic "click"-type cycloaddition chemistry with e.g. a new thiazole orange azide combined with thiazole red yields dual emitting DNA probes with good fluorescence readout properties.
通过后合成的“点击”型环加成化学,将菁染料叠氮化物作为能量供体染料插入,例如与新的噻唑橙叠氮化物结合的噻唑红,得到具有良好荧光读出性能的双发射 DNA 探针。