Hurley M M, Martin D L, Kream B E, Raisz L G
Department of Medicine, University of Connecticut Health Center, Farmington 06032.
Bone. 1990;11(1):47-51. doi: 10.1016/8756-3282(90)90071-6.
We tested the effect of ethanol and its metabolite, acetaldehyde, on bone formation as measured by [3H]proline incorporation into collagenase digestible protein (CDP) and noncollagen protein (NCP), and on DNA synthesis as measured by [3H]thymidine (TdR) incorporation in fetal rat calvaria. We also determined the effects of ethanol and acetaldehyde on prostaglandin E2 (PGE2) release from calvaria and on bone resorption as measured by 45Ca release from fetal rat long bones. Bones were cultured in multiwell plastic dishes (open system) or in stoppered Erlenmeyer flasks (closed system) for 24 to 96 h. In the open system, 1% ethanol (v/v; 172 mM) resulted in a 31% decrease in TdR incorporation at 24 h with no effect on CDP and NCP. At 0.1% (17.2 mM), ethanol increased TdR by 22%, CDP by 73% and NCP by 67% at 24 h, but these effects were not sustained at 96 h. At 24 h, 1% and 0.3% ethanol decreased PGE2 release by 88% and 75% respectively. This effect was sustained for 96 h only at the higher concentration. In the closed system, 0.1% ethanol increased TdR incorporation by 38% at 24 h. However, there was no effect on the labeling of CDP or NCP. Because its boiling point is 21 degrees C, acetaldehyde could only be tested in the closed system. Acetaldehyde markedly inhibited bone metabolism. At 24 h, 0.003% (0.54 mM) to 0.01% (1.79 mM) acetaldehyde caused a dose-related inhibition of TdR incorporation from 23 to 45%. At 0.01% and 0.03% acetaldehyde inhibited proline incorporation into CDP by 48% and 94% and NCP by 40% and 74% respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
我们通过检测[3H]脯氨酸掺入胶原酶可消化蛋白（CDP）和非胶原蛋白（NCP）的情况，来测试乙醇及其代谢产物乙醛对骨形成的影响；通过检测[3H]胸腺嘧啶核苷（TdR）掺入胎鼠颅骨的情况，来测试其对DNA合成的影响。我们还测定了乙醇和乙醛对颅骨中前列腺素E2（PGE2）释放的影响，以及通过检测胎鼠长骨中45Ca的释放来测定其对骨吸收的影响。将骨骼置于多孔塑料培养皿（开放系统）或带塞锥形瓶（封闭系统）中培养24至96小时。在开放系统中，1%乙醇（体积/体积；172 mM）在24小时时导致TdR掺入量降低31%，对CDP和NCP无影响。在0.1%（17.2 mM）时，乙醇在24小时时使TdR增加22%，CDP增加73%，NCP增加67%，但在96小时时这些作用未持续。在24小时时，1%和0.3%乙醇分别使PGE2释放降低88%和75%。仅在较高浓度下，这种作用持续96小时。在封闭系统中，0.1%乙醇在24小时时使TdR掺入量增加38%。然而，对CDP或NCP的标记无影响。由于乙醛沸点为21℃，故只能在封闭系统中进行测试。乙醛显著抑制骨代谢。在24小时时，0.003%（0.54 mM）至0.01%（1.79 mM）乙醛导致TdR掺入量呈剂量相关抑制，从23%降至45%。在0.01%和0.03%时，乙醛分别使脯氨酸掺入CDP降低48%和94%，使脯氨酸掺入NCP降低40%和74%。（摘要截断于250字）
