Palanisamy Nallasamy, Mohan Dass Selvam
Department of Biology, The University of North Carolina at Greensboro, Greensboro, NC 27402-6170, USA.
J Basic Clin Physiol Pharmacol. 2013;24(1):51-7. doi: 10.1515/jbcpp-2012-0038.
The present study aimed to determine the effect of Trichopus zeylanicus leaf extract (TZ Et) on mercuric chloride (HgCl2)-induced liver toxicity in rats.
Wistar albino male rats were divided into four groups. Group I was administered physiological saline as a vehicle control. Group II was administered HgCl2 (0.4 mg/kg body weight/day) intraperitoneally for 20 days. Groups III and IV were administered TZ Et orally (100 mg/kg body weight/day) for 20 days with or without intraperitoneal administration of HgCl2. Serum hepatic marker enzymes, enzymic and non-enzymic antioxidants, liver functional markers, lipid peroxidation, and liver histology were evaluated.
The toxic effect of HgCl2 was indicated by the significantly (p<0.05) increased serum marker enzymes, liver functional markers, and lipid peroxidation. The activities of enzymic and non-enzymic antioxidants were significantly (p<0.05) decreased. TZ Et significantly (p<0.05) prevented these pathological alterations as observed by histology.
The present study concludes that TZ Et has the ability to alleviate the hepatotoxic effects caused by HgCl2 in rats.
本研究旨在确定锡兰肉豆蔻叶提取物（TZ Et）对氯化汞（HgCl2）诱导的大鼠肝脏毒性的影响。
将雄性Wistar白化大鼠分为四组。第一组给予生理盐水作为溶剂对照。第二组腹腔注射HgCl2（0.4 mg/kg体重/天），持续20天。第三组和第四组分别在腹腔注射或不注射HgCl2的情况下，口服TZ Et（100 mg/kg体重/天），持续20天。评估血清肝标志物酶、酶性和非酶性抗氧化剂、肝功能标志物、脂质过氧化和肝脏组织学。
血清标志物酶、肝功能标志物和脂质过氧化显著（p<0.05）升高，表明HgCl2具有毒性作用。酶性和非酶性抗氧化剂的活性显著（p<0.05）降低。组织学观察表明，TZ Et显著（p<0.05）预防了这些病理改变。
本研究得出结论，TZ Et具有减轻HgCl2对大鼠肝脏毒性作用的能力。
