Department of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Iran.
Colloids Surf B Biointerfaces. 2013 Apr 1;104:186-93. doi: 10.1016/j.colsurfb.2012.12.011. Epub 2012 Dec 21.
A novel electrochemical sensor for the simultaneous determination of glutathione (GSH) and nicotinamide adenine dinucleotide (NADH) is described. The sensor is based on a carbon paste electrode (CPE) modified with benzamide derivative and multiwall carbon nanotubes. This mixture makes a modified electrode that is sensitive for the electrochemical detection of these compounds. Under optimum conditions and at pH 7.0, oxidation of GSH occurs at a potential of about 330 mV less positive than that at an unmodified CPE. The voltammetric peak currents are linearly dependent on GSH and NADH concentrations in the ranges 0.09-300 μmol L(-1) GSH and 5.0-600 μmol L(-1) NADH. The detection limits found for GSH and NADH were 0.05 μmol L(-1) and 1.0 μmol L(-1), respectively. The electrochemical sensor was also used for the determination of GSH in urine, pharmaceutical and hemolysed erythrocyte samples.
一种用于同时测定谷胱甘肽（GSH）和烟酰胺腺嘌呤二核苷酸（NADH）的新型电化学传感器。该传感器基于碳糊电极（CPE），用苯甲酰胺衍生物和多壁碳纳米管进行修饰。这种混合物使得修饰电极对这些化合物的电化学检测具有敏感性。在最佳条件下，在 pH 7.0 时，GSH 的氧化在比未修饰的 CPE 更正的约 330 mV 处发生。在 GSH 和 NADH 的浓度范围内为 0.09-300 μmol L(-1) GSH 和 5.0-600 μmol L(-1) NADH 时，伏安峰电流呈线性依赖关系。GSH 和 NADH 的检测限分别为 0.05 μmol L(-1) 和 1.0 μmol L(-1)。电化学传感器还用于测定尿液、药物和溶血红细胞样品中的 GSH。
