School of Life Science, Shandong University of Technology, Zibo, 255049, Shandong, China.
Mol Biol Rep. 2013 Jun;40(6):4075-81. doi: 10.1007/s11033-012-2485-z. Epub 2013 Jan 12.
MicroRNAs (miRNAs), which are 18 ~ 24 nucleotides length, play important roles in regulating the expression of gene at the post-transcription level. Dugesia japonica is a branch of planarian organism. It is a model organism for studying the role of miRNAs in stem cell function. Next generation sequencing technology was used to identify the miRNAs of D. japonica. Bioinformatic analysis showed that 262 miRNA and miRNA* sequences were discovered, of which 102 miRNAs were the same as Schmidtea mediterranea and 160 miRNAs were related to other animals. There were 21 miRNAs expressed differentially after amputation. Results also revealed that some key miRNAs might play essential roles in the regeneration progress and some miRNAs might take part in the regulation progress of polarity regeneration in D. japonica.
微小 RNA（miRNAs）是一类长度为 18~24 个核苷酸的非编码 RNA，在转录后水平调控基因的表达中发挥着重要作用。日本三角涡虫是扁形动物门的一个分支，是研究 miRNAs 在干细胞功能中的作用的模式生物。本研究利用下一代测序技术对日本三角涡虫的 miRNAs 进行鉴定，生物信息学分析共发现 262 个 miRNA 和 miRNA*序列，其中 102 个 miRNA 与 Schmidtea mediterranea 相同，160 个 miRNA 与其他动物相关。在截肢后有 21 个 miRNA 表达差异。结果表明，一些关键的 miRNAs 可能在再生过程中发挥重要作用，而一些 miRNAs 可能参与了日本三角涡虫极性再生的调控过程。
