Laboratory of Wild Animal Biology and Medicine, Federal University of Pará, Pará, Brazil.
Reprod Sci. 2013 Aug;20(8):990-7. doi: 10.1177/1933719112472737. Epub 2013 Jan 11.
The aim of this study was to evaluate whether an in vitro culture (IVC) medium containing either or not β-mercaptoethanol (BME), bone morphogenetic protein 4 (BMP4), or pregnant mare serum gonadotrophin (PMSG) could be able to promote the development of capuchin monkeys' preantral follicles enclosed in ovarian cortical strips. Follicular viability after IVC was similar to control (89.32%). Primordial follicle recruitment to primary stage was not reached with IVC, but the rate of secondary follicle formation was increased in the medium supplemented with BME, BMP4, and PMSG (44.86%) when compared to IVC control (9.20%). In the medium supplemented with BME, BMP4, and PMSG, contrary to other media, anti-müllerian hormone-messenger RNA (mRNA) expression in ovarian tissue was upregulated (3.4-fold), while that of growth differentiation factor-9 was maintained. The BMP4-mRNA expression, however, appeared downregulated in all cultured tissues. Our findings show a favorable effect of BME, BMP4, and PMSG on the in vitro development of secondary follicles from capuchin monkeys.
本研究旨在评估含有β-巯基乙醇（BME）、骨形态发生蛋白 4（BMP4）或孕马血清促性腺激素（PMSG）的体外培养（IVC）介质是否能够促进封闭在卵巢皮质带中的食蟹猴原始卵泡的发育。IVC 后卵泡的活力与对照组相似（89.32%）。IVC 未能招募原始卵泡进入初级阶段，但在补充 BME、BMP4 和 PMSG 的培养基中，次级卵泡的形成率增加（44.86%），而 IVC 对照组仅为 9.20%。在补充 BME、BMP4 和 PMSG 的培养基中，与其他培养基相反，卵巢组织中的抗苗勒管激素信使 RNA（mRNA）表达上调（3.4 倍），而生长分化因子 9 的表达则保持不变。然而，所有培养组织中的 BMP4-mRNA 表达似乎都下调了。我们的研究结果表明，BME、BMP4 和 PMSG 对食蟹猴次级卵泡的体外发育有良好的影响。
