CT10、CT45 和 GAGE7 抗原在人乳腺癌中的异质性表达及其预后意义。

Heterogeneous expression of CT10, CT45 and GAGE7 antigens and their prognostic significance in human breast carcinoma.

机构信息

Fourth Military Medical University, No. 17 Changle West Road, Xi'an, Shaanxi, P.R. China.

出版信息

Jpn J Clin Oncol. 2013 Mar;43(3):243-50. doi: 10.1093/jjco/hys236. Epub 2013 Jan 11.

DOI:10.1093/jjco/hys236

PMID:23315387

Abstract

OBJECTIVE

The goal of this study was to detect the intertumoral heterogeneity of CT10, CT45 and GAGE7 expression and further to analyze their prognostic value.

METHODS

The intertumoral heterogeneity of three cancer/testis antigens was examined by immunohistochemistry using 120 samples from patients with infiltrating ductal breast carcinoma. The expression patterns were classified and correlated with the clinicopathologic variables and outcome of the patients.

RESULTS

CT10 showed punctate, focal and diffuse expression patterns according to the characteristic of its distribution. CT45 showed cytoplasmic, nuclear or combined cytoplasmic and nuclear expression patterns according to its subcellular location. GAGE7 exhibited nuclear, cytoplasmic and nucleolar expression patterns. Three cancer/testis antigens were also observed coordinately expressed in infiltrating ductal breast carcinoma. Patients with tumors with CT10 expression was significantly correlated with nodal metastases (P < 0.001) and advanced clinical stages (P = 0.001). Patients with tumors with cytoplasmic GAGE7 and with the expression of two or more cancer/testis antigens were significantly correlated with advanced clinical stages (P = 0.001 and P = 0.030). No signiﬁcant difference was identiﬁed between the different expression patterns of CT45 and clinicopathologic variables. In addition, Kaplan-Meier analysis revealed that diffuse CT10 expression and coexpression of three cancer/testis antigens were related to the poor prognosis of patients with infiltrating ductal breast carcinoma.

CONCLUSIONS

Diffuse CT10 expression and the coexpression of three cancer/testis antigens can be used as a biomarker to distinguish patients with a poorer outcome of the breast carcinoma. Our finding may provide useful data for evaluating the prognosis of this disease and improving the effectiveness of therapeutic application based on the three cancer/testis antigens.

摘要

目的

本研究旨在检测 CT10、CT45 和 GAGE7 表达的肿瘤间异质性，并进一步分析其预后价值。

方法

使用 120 例浸润性导管乳腺癌患者的样本，通过免疫组织化学方法检测三种肿瘤/睾丸抗原的肿瘤间异质性。将表达模式进行分类，并与患者的临床病理变量和结局相关联。

结果

根据其分布特征，CT10 显示点状、局灶性和弥漫性表达模式。根据其亚细胞定位，CT45 显示细胞质、核或细胞质和核的混合表达模式。GAGE7 显示核、细胞质和核仁表达模式。在浸润性导管乳腺癌中还观察到三种肿瘤/睾丸抗原的协调表达。CT10 表达的肿瘤患者与淋巴结转移（P<0.001）和临床晚期（P=0.001）显著相关。细胞质 GAGE7 表达和两种或两种以上肿瘤/睾丸抗原表达的肿瘤患者与临床晚期显著相关（P=0.001 和 P=0.030）。CT45 的不同表达模式与临床病理变量之间无显著差异。此外，Kaplan-Meier 分析显示弥漫性 CT10 表达和三种肿瘤/睾丸抗原的共表达与浸润性导管乳腺癌患者的不良预后相关。