原发性中枢神经系统肿瘤中的催乳素基因表达。
Prolactin gene expression in primary central nervous system tumors.
作者信息
Mendes Graziella Alebrant, Pereira-Lima Júlia Fernanda Semmelmann, Kohek Maria Beatriz, Trott Geraldine, Di Domenico Marlise, Ferreira Nelson Pires, Oliveira Miriam da Costa
机构信息
Postgraduate Program in Pathology, Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, Brazil.
出版信息
J Negat Results Biomed. 2013 Jan 14;12(1):4. doi: 10.1186/1477-5751-12-4.
BACKGROUND
Prolactin (PRL) is a hormone synthesized in both the pituitary gland and extrapituitary sites. It has been associated with the occurrence of neoplasms and, more recently, with central nervous system (CNS) neoplasms. The aim of this study was to evaluate prolactin expression in primary central nervous system tumors through quantitative real-time PCR and immunohistochemistry (IH).
RESULTS
Patient mean age was 49.1 years (SD 15.43), and females accounted for 70% of the sample. The most frequent subtype of histological tumor was meningioma (61.5%), followed by glioblastoma (22.9%). Twenty cases (28.6%) showed prolactin expression by immunohistochemistry, most of them females (18 cases, 90%). Quantitative real-time PCR did not show any prolactin expression.
CONCLUSIONS
Despite the presence of prolactin expression by IH, the lack of its expression by quantitative real-time PCR indicates that its presence in primary tumors in CNS is not a reflex of local production.
背景
催乳素（PRL）是一种在垂体和垂体外部位合成的激素。它与肿瘤的发生有关，最近还与中枢神经系统（CNS）肿瘤有关。本研究的目的是通过定量实时PCR和免疫组织化学（IH）评估原发性中枢神经系统肿瘤中催乳素的表达。
结果
患者平均年龄为49.1岁（标准差15.43），女性占样本的70%。最常见的组织学肿瘤亚型是脑膜瘤（61.5%），其次是胶质母细胞瘤（22.9%）。20例（28.6%）通过免疫组织化学显示催乳素表达，其中大多数为女性（18例，90%）。定量实时PCR未显示任何催乳素表达。
结论
尽管免疫组织化学显示存在催乳素表达，但定量实时PCR缺乏其表达表明其在中枢神经系统原发性肿瘤中的存在并非局部产生的反映。
Zotero 插件