Wang Ting-Ting, Wang Shao-Kang, Huang Gui-Ling, Sun Gui-Ju
Key Laboratory of Environmental Medicine and Engineering of Ministry of Education, and Department of Nutrition and Food Hygiene, School of Public Health, Southeast University, Nanjing, China.
Asian Pac J Cancer Prev. 2012;13(11):5455-61. doi: 10.7314/apjcp.2012.13.11.5455.
Luteolin is a plant flavonoid which exhibits anti-oxidative, anti-inflammatory and anti-tumor effects. However, the antiproliferative potential of luteolin is not fully understood. In this study, we investigated the effect of luteolin on cell cycling and apoptosis in human esophageal squamous carcinoma cell line Eca109 cells. MTT assays showed that luteolin had obvious cytotoxicity on Eca109 with an IC50 of 70.7±1.72 μM at 24 h. Luteolin arrested cell cycle progression in the G0/G1 phase and prevented entry into S phase in a dose- and time-dependent manner. as assessed by FCM. Luteolin induced apoptosis of Eca109 cells was demonstrated by AO/EB staining assay and annexin V-FITC/PI staining. Moreover, luteolin downregulated the expression of cyclin D1, survivin and c-myc, and it also upregulated the expression of p53, in line with the fact that luteolin was able to inhibit Eca109 cell proliferation.
木犀草素是一种植物类黄酮，具有抗氧化、抗炎和抗肿瘤作用。然而，木犀草素的抗增殖潜力尚未完全明确。在本研究中，我们研究了木犀草素对人食管鳞状癌细胞系Eca109细胞周期和凋亡的影响。MTT试验表明，木犀草素对Eca109细胞具有明显的细胞毒性，24小时时的IC50为70.7±1.72μM。通过流式细胞术评估，木犀草素以剂量和时间依赖性方式使细胞周期进程停滞在G0/G1期，并阻止细胞进入S期。AO/EB染色试验和膜联蛋白V-FITC/PI染色证明木犀草素诱导了Eca109细胞凋亡。此外，木犀草素下调了细胞周期蛋白D1、生存素和c-myc的表达，同时上调了p53的表达，这与木犀草素能够抑制Eca109细胞增殖的事实相符。
