Department of Chemistry and Chemical Engineering, Xi'an University of Arts and Science, Xi'an, Shaanxi 710069, China.
Appl Spectrosc. 2013 Jan;67(1):106-11. doi: 10.1366/12-06760.
Ergosta-4,6,8(14),22-tetraen-3-one (ergone) isolated from Polyporus umbellatus possesses a variety of pharmacological activities in vivo and in vitro, including cytotoxic, diuretic, and immunosuppressive effect. The interaction of cerium ions (Ce(3+)) with ergone was studied by fluorescence and absorption spectroscopy. Spectra data revealed that Ce(3+) ions exhibited emission maxima around 350 nm when the excitation wavelength was fixed at 255 or 290 nm, and the fluorescence of Ce(3+) ions was quenched by the addition of ergone, indicating that a Ce(3+)-ergone complex was formed. According to the modified Benesi-Hildebrand equation, the binding constant of interaction of Ce(3+) ions with ergone was obtained at room temperature. Based on this, a sensitive spectrofluorometric method using Ce(3+) ions as a probe was applied for the identification and quantification of ergone in rat plasma, feces, and urine. The linear ranges of the calibration curves were 1.31 to 4.50 μM for plasma, 1.12-9.87 μM for feces, and 1.28-3.42 μM for urine, and the ergone recoveries were found to be 97.1 ± 0.9%, 98.2 ± 0.7% and 96.5 ± 1.4% for plasma, feces, and urine, respectively. The intraday and inter-day relative standard deviations were less than 9.7%. The proposed spectrofluorometric method is simple and rapid for the quantitative determination of ergone in rat plasma, feces, and urine, and it is affordable for most laboratories because it has few requirements and uses low cost, easy to operate equipment.
从香菇中分离得到的麦角甾-4,6,8(14),22-四烯-3-酮（ergone）具有多种体内和体外的药理活性，包括细胞毒性、利尿和免疫抑制作用。本文采用荧光和吸收光谱法研究了铈离子（Ce(3+))与 ergone 的相互作用。光谱数据表明，当激发波长固定在 255 或 290nm 时，Ce(3+)离子在 350nm 左右表现出发射最大值，并且 ergone 的加入使 Ce(3+)离子的荧光猝灭，表明形成了 Ce(3+)-ergone 配合物。根据改进的 Benesi-Hildebrand 方程，在室温下获得了 Ce(3+)离子与 ergone 相互作用的结合常数。基于此，应用 Ce(3+)离子作为探针，建立了一种灵敏的荧光分光光度法，用于鉴定和定量大鼠血浆、粪便和尿液中的 ergone。血浆中校准曲线的线性范围为 1.31-4.50μM，粪便中为 1.12-9.87μM，尿液中为 1.28-3.42μM，血浆、粪便和尿液中 ergone 的回收率分别为 97.1±0.9%、98.2±0.7%和 96.5±1.4%。日内和日间相对标准偏差均小于 9.7%。该荧光分光光度法简单快速，可用于定量测定大鼠血浆、粪便和尿液中的 ergone，并且由于其要求低，使用成本低，操作简单，大多数实验室都负担得起。
