恩度联合顺铂对 Lewis 肺癌大鼠 VEGF 和 Sema3A 表达的影响。

Effect of endostar combined with cisplatin on expression of VEGF and Sema3A of Lewis lung cancer rats.

机构信息

Master of Respiratory Medicine, Hebei Northern College, Zhangjiakou 075000, Hebei, China.

出版信息

Asian Pac J Trop Med. 2013 Jan;6(1):57-60. doi: 10.1016/S1995-7645(12)60201-6.

DOI:10.1016/S1995-7645(12)60201-6

PMID:23317887

Abstract

OBJECTIVE

To evaluate the therapeutic effect of endostar (ED) combined with cisplatin(DDP) on model of C57BL/6 rats, and to further investigate the inhibiting mechanism of endostar from tumor angiogenesis.

METHODS

Lewis lung cancer cells were inoculated in C57BL/6 mouse, then the mouse were randomized into control group (group A), ED (group B), DDP (group C) and ED/DDP (group D). They were treated according to the plan. And the expressions of VEGF and Sema3A were evaluated by immunhistochemisty.

RESULTS

The weight of tumor increased in group A and B. It was decreased in group C and D. The tumor volume was increased in all the 4 groups. The VEGF expression of group D was obviously lower than the other group 3, but the Sema3A expressed of group D was significantly strengthener than the other group 3. The VEGF expression of group B and group D were obviously low especially in the 4th-8th days. Pearson correlated analysis showed that the expression VEGF and Sema3A were negatively correlated (r=-0.72, P<0.05).

CONCLUSIONS

ED combined with DDP could control the tumor growth effectively, and avoid weight loss. ED could reduce VEGF expression, and enhance Sema3A expression. Tumor vessel presents transient normalization. It is easy for DDP perfusion, and to kill tumor cells.

摘要

目的

评价恩度（ED）联合顺铂（DDP）治疗 C57BL/6 大鼠模型的疗效，并进一步探讨恩度抑制肿瘤血管生成的机制。

方法

将 Lewis 肺癌细胞接种于 C57BL/6 小鼠，随机分为对照组（A 组）、ED 组（B 组）、DDP 组（C 组）和 ED/DDP 组（D 组），按照方案进行治疗，免疫组化法检测 VEGF 和 Sema3A 的表达。

结果

A 组和 B 组肿瘤重量增加，C 组和 D 组肿瘤重量降低。四组肿瘤体积均增加，D 组 VEGF 表达明显低于其他三组，但 D 组 Sema3A 表达明显增强。B 组和 D 组 VEGF 表达在第 4-8 天明显降低。Pearson 相关分析显示，VEGF 和 Sema3A 的表达呈负相关（r=-0.72，P<0.05）。