Liu Dan, Li Yang, Wang Xuelin, Wang Yanjun, Ma Yinrui, Chen Yanwen, Ming Haixia
Gansu University of Chinese Medicine, Institute of Integrative Traditional Chinese and Western Medicine, Gansu University of Traditional Chinese Medicine, Provincial Key Laboratory of Molecular Medicine and Prevention and Treatment of Major Diseases with Traditional Chinese Medicine in Gansu Colleges and Universities, Lanzhou 730000, China.
Gansu University of Chinese Medicine, Lanzhou 730000, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2018 Dec;34(12):1105-1110.
Objective To investigate the effects of Astragalus polysaccharide (APS) combined with cisplatin (DDP) on the pathological morphology of recurrent tumor and the expression of metastasis-related proteins CD44, CD62P and osteopontin (OPN) following Lewis lung carcinoma (LLC) surgery. Methods LLC cell suspension was injected subcutaneously into palmula of left hind limb of C57BL/6J mice as a tumor-supply group. The other 80 mice were randomized into 8 groups: model group, APS-treated groups at different concentrations of 50, 100 and 200 μg/mL, 6 mg/kg DDP-treated group, and 3 mg/kg DDP combined with 50, 100, 200 μg/mL APS-treated groups. The palmula tumor cells were collected from the tumor-supply group 10 days after LLC injection and then injected subcutaneously into all of the 80 mice to establish the recurrent and metastatic mouse models of lung cancer. Subsequently, corresponding different substances were administrated intraperitoneally in the different treated groups since the next day. After 15 days' administration, all the mice were sacrificed by cervical spine dislocation. Morphological characteristics were observed by H&E staining, and the protein expression of CD44, CD62P and OPN were measured by immunohistochemistry. Results Compared with the model group, the pathological changes of the recurrent tissues in each treatment group were alleviated to some extent, especially in the DDP combined with 200 μg/mL APS group; the expression of CD44, CD62P and OPN in each treatment group decreased, especially in the DDP group and DDP combined with 100 and 200 μg/mL APS-treated groups. Conclusion APS combined with DDP could significantly inhibit the growth and metastasis of Lewis lung cancer cells, which might be associated with the reduced expression of CD44, CD62P and OPN.
目的 探讨黄芪多糖（APS）联合顺铂（DDP）对Lewis肺癌（LLC）术后复发肿瘤病理形态及转移相关蛋白CD44、CD62P和骨桥蛋白（OPN）表达的影响。方法 将LLC细胞悬液皮下注射到C57BL/6J小鼠左后肢掌部作为肿瘤供体组。将另外80只小鼠随机分为8组：模型组、50、100和200μg/mL不同浓度APS处理组、6mg/kg DDP处理组、3mg/kg DDP联合50、100、200μg/mL APS处理组。在LLC注射10天后从肿瘤供体组收集掌部肿瘤细胞，然后皮下注射到所有80只小鼠体内，建立肺癌复发转移小鼠模型。随后，从次日起对不同处理组腹腔注射相应不同物质。给药15天后，所有小鼠经颈椎脱臼处死。通过苏木精-伊红（H&E）染色观察形态学特征，采用免疫组织化学法检测CD44、CD62P和OPN的蛋白表达。结果 与模型组相比，各治疗组复发组织的病理变化均有不同程度减轻，尤其是DDP联合200μg/mL APS组；各治疗组CD44、CD62P和OPN的表达均降低，尤其是DDP组以及DDP联合100和200μg/mL APS处理组。结论 APS联合DDP可显著抑制Lewis肺癌细胞的生长和转移，这可能与CD44、CD62P和OPN表达降低有关。
