葡萄球菌 petrasii 新种，包括 S. petrasii 亚种 petrasii 亚种新种和 S. petrasii 亚种 croceilyticus 亚种新种，从人体临床标本和人类耳部感染中分离得到。
Staphylococcus petrasii sp. nov. including S. petrasii subsp. petrasii subsp. nov. and S. petrasii subsp. croceilyticus subsp. nov., isolated from human clinical specimens and human ear infections.
机构信息
Division of Genetics and Molecular Biology, Department of Experimental Biology, Faculty of Science, Masaryk University, Kotlářská 2, 611 37 Brno, Czech Republic.
出版信息
Syst Appl Microbiol. 2013 Mar;36(2):90-5. doi: 10.1016/j.syapm.2012.11.004. Epub 2013 Jan 12.
Thirteen coagulase-negative, oxidase-negative, and novobiocin-susceptible staphylococci were isolated from human clinical specimens. The isolates were differentiated from known staphylococcal species on the basis of 16S rRNA, hsp60, rpoB, dnaJ, tuf, and gap gene sequencing, automated ribotyping, (GTG)5-PCR fingerprinting, and MALDI-TOF MS analysis. Phylogenetic analysis based on the 16S rRNA gene sequence indicated phylogenetic relatedness of the analyzed strains to Staphylococcus haemolyticus, Staphylococcus hominis, Staphylococcus devriesei, and Staphylococcus lugdunensis. DNA-DNA hybridization experiments between representative strains CCM 8418(T), CCM 8421(T), and the closest phylogenetic neighbors confirmed that the isolates represent novel Staphylococcus species, for which the name Staphylococcus petrasii sp. nov. is proposed. Genotypic and phenotypic analyses unambiguously split the strains into two closely related subclusters. Based on the results, two novel subspecies S. petrasii subsp. petrasii subsp. nov. and S. petrasii subsp. croceilyticus subsp. nov. are proposed, with type strains CCM 8418(T) (=CCUG 62727(T)) and CCM 8421(T) (=CCUG 62728(T)), respectively.
从人类临床标本中分离出 13 株凝固酶阴性、氧化酶阴性、新生霉素敏感的葡萄球菌。根据 16S rRNA、hsp60、rpoB、dnaJ、tuf 和 gap 基因测序、自动核糖体分型、(GTG)5-PCR 指纹图谱和 MALDI-TOF MS 分析，将这些分离株与已知的葡萄球菌种区分开来。基于 16S rRNA 基因序列的系统发育分析表明，分析菌株与溶血葡萄球菌、人葡萄球菌、德氏葡萄球菌和路邓葡萄球菌具有系统发育相关性。代表菌株 CCM 8418(T)、CCM 8421(T) 与最接近的系统发育近缘种之间的 DNA-DNA 杂交实验证实，这些分离株代表了一种新的葡萄球菌种，建议将其命名为 Petras 葡萄球菌。基因型和表型分析明确将这些菌株分为两个密切相关的亚群。基于这些结果，提出了两个新的亚种，Petras 葡萄球菌亚种。petrasii 亚种。nov. 和 Petras 葡萄球菌亚种。croceilyticus 亚种。nov.，其模式菌株分别为 CCM 8418(T)(=CCUG 62727(T))和 CCM 8421(T)(=CCUG 62728(T))。
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