Švec Pavel, Bel Annelies De, Sedláček Ivo, Petráš Petr, Gelbíčová Tereza, Černohlávková Jitka, Mašlanˇová Ivana, Cnockaert Margo, Varbanovová Ivana, Echahidi Fedoua, Vandamme Peter, Pantu Ček Roman
Czech Collection of Microorganisms, Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 5, Bld. A25, 625 00 Brno, Czech Republic.
Department of Microbiology and Infection Control, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel (VUB), Laarbeeklaan 101, 1090 Brussels, Belgium.
Int J Syst Evol Microbiol. 2015 Jul;65(7):2071-2077. doi: 10.1099/ijs.0.000220. Epub 2015 Mar 31.
Seven coagulase-negative, oxidase-negative and novobiocin-susceptible staphylococci assigned tentatively as Staphylococcus petrasii were investigated in this study in order to elucidate their taxonomic position. All strains were initially shown to form a genetically homogeneous group separated from remaining species of the genus Staphylococcus by using a repetitive sequence-based PCR fingerprinting with the (GTG)5 primer. Phylogenetic analysis based on 16S rRNA gene, hsp60, rpoB, dnaJ, gap and tuf sequences showed that the group is closely related to Staphylococcus petrasii but separated from the three hitherto known subspecies, S. petrasii subsp. petrasii, S. petrasii subsp. croceilyticus and S. petrasii subsp. jettensis. Further investigation using automated ribotyping, MALDI-TOF mass spectrometry, fatty acid methyl ester analysis, DNA-DNA hybridization and extensive biotyping confirmed that the analysed group represents a novel subspecies within S. petrasii, for which the name Staphylococcus petrasii subsp. pragensis subsp. nov. is proposed. The type strain is NRL/St 12/356(T) ( = CCM 8529(T) = LMG 28327(T)).
为阐明七株凝固酶阴性、氧化酶阴性且对新生霉素敏感的葡萄球菌（暂定为佩氏葡萄球菌）的分类地位，开展了本研究。通过使用基于重复序列的PCR指纹图谱技术（采用(GTG)5引物），所有菌株最初均显示形成一个与葡萄球菌属其余菌种分离的遗传同质性群体。基于16S rRNA基因、hsp60、rpoB、dnaJ、gap和tuf序列的系统发育分析表明，该群体与佩氏葡萄球菌密切相关，但与迄今已知的三个亚种（佩氏葡萄球菌佩氏亚种、佩氏葡萄球菌溶黄亚种和佩氏葡萄球菌杰氏亚种）相分离。使用自动核糖体分型、基质辅助激光解吸电离飞行时间质谱分析、脂肪酸甲酯分析、DNA-DNA杂交以及广泛的生化分型进行的进一步研究证实，所分析的群体代表佩氏葡萄球菌内的一个新亚种，为此提议将其命名为佩氏葡萄球菌布拉格亚种（Staphylococcus petrasii subsp. pragensis subsp. nov.）。模式菌株为NRL/St 12/356(T)（= CCM 8529(T) = LMG 28327(T)）。
