Eschrich K, Schellenberger W, Hofmann E
Acta Biol Med Ger. 1979;38(11-12):K25-33.
The dynamic pattern of an open reconstituted in-vitro enzyme system containing phosphofructokinase, pyruvate kinase and adenylate kinase has been investigated. The approach is experimentally based on a stirred flow-through reactor. Stationary concentrations of phosphofructokinase and pyruvate kinase are maintained by entrapment in polyacrylamide gel particles. The results fit to a theoretical model based on the initial kinetic responses of the enzymes involved. An S-shaped steady state input characteristic for fructose 6-phosphate has been experimentally obtained.
对含有磷酸果糖激酶、丙酮酸激酶和腺苷酸激酶的开放式重组体外酶系统的动态模式进行了研究。该方法基于搅拌式流通反应器进行实验。通过包埋在聚丙烯酰胺凝胶颗粒中来维持磷酸果糖激酶和丙酮酸激酶的固定浓度。结果符合基于所涉及酶的初始动力学响应的理论模型。通过实验获得了6-磷酸果糖的S形稳态输入特征。
