Department of Materials Science and Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan.
Colloids Surf B Biointerfaces. 2013 Apr 1;104:221-8. doi: 10.1016/j.colsurfb.2012.11.040. Epub 2012 Dec 8.
A rapid and efficient tryptic proteolysis approach has been developed by combining graphene with near infrared (NIR)-assisted protein digestion. Based on the unique properties of graphene such as strong absorption ability of electromagnetic radiation over a wide range of wavelengths and excellent thermal conductivity, we developed a novel fast NIR-assisted tryptic digestion method using graphene, allowing the entire time for identification of protein to be greatly reduced. Bovine serum albumin (BSA), myoglobin (Myo), lysozyme (Lys) and cytochrome c (Cyt-c) were denatured with a digestion time as short as 3s. Peptide fragments were identified by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS). Equivalent or better digestion efficiency was observed comparing to current in-solution digestion methods. This novel graphene-based NIR-assisted digestion approach may provide a promising proteomic identification technique to biomedical and clinical research.
一种快速高效的胰蛋白酶切方法已经通过将石墨烯与近红外（NIR）辅助蛋白消化相结合而开发出来。基于石墨烯的独特性质，如对宽波长范围的电磁辐射的强吸收能力和优异的导热性，我们开发了一种使用石墨烯的新型快速 NIR 辅助胰蛋白酶消化方法，使鉴定蛋白质的整个时间大大缩短。牛血清白蛋白（BSA）、肌红蛋白（Myo）、溶菌酶（Lys）和细胞色素 c（Cyt-c）的变性消化时间短至 3 秒。肽片段通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）进行鉴定。与目前的溶液内消化方法相比，观察到等效或更好的消化效率。这种基于石墨烯的新型 NIR 辅助消化方法可能为生物医学和临床研究提供一种有前途的蛋白质组鉴定技术。
