脑突触体Ca2+摄取：Sprague-Dawley、Wistar-Kyoto和自发性高血压大鼠的比较

Brain synaptosomal Ca2+ uptake: comparison of Sprague-Dawley, Wistar-Kyoto and spontaneously hypertensive rats.

Honda H, Shibuya T, Salafsky B

Department of Biomedical Sciences, University of Illinois College of Medicine, Rockford 61107-1897.

Comp Biochem Physiol B. 1990;95(3):555-8. doi: 10.1016/0305-0491(90)90020-t.

Abstract

K(+)-stimulated 45Ca2+ uptake by synaptosomes was measured with respect to the strain differences between Sprague-Dawley (SD), Wistar-Kyoto (WKY) and spontaneously hypertensive rats (SHR). 2. 45Ca2+ uptake by synaptosomes isolated from cerebral cortex of SD, WKY and SHR was measured at 15, 30, 60, 120 and 240 sec time periods. 3. The sequence of both the magnitude and rate of resting and depolarization-dependent 45Ca2+ uptake was SHR greater than WKY greater than SD. 4. The fastest rates of resting and depolarization-dependent 45Ca2+ uptake occurred in each rat during the first 15 sec and uptake rates dropped off quickly in both resting and depolarization states. 5. At 15 sec, there were significant differences between SHR and WKY, while there were no significant differences between WKY and SD. 6. The results suggest that an important alteration in Ca2+ channel characteristics may occur in SHR brain synaptosomes.

摘要

针对斯普拉格-道利大鼠（SD）、威斯塔-京都大鼠（WKY）和自发性高血压大鼠（SHR）之间的品系差异，测定了突触体对K(+)刺激的45Ca2+摄取量。2. 在15、30、60、120和240秒的时间段内，测定了从SD、WKY和SHR的大脑皮层分离出的突触体对45Ca2+的摄取量。3. 静息和去极化依赖性45Ca2+摄取的量和速率顺序均为SHR大于WKY大于SD。4. 每只大鼠在最初15秒内静息和去极化依赖性45Ca2+摄取的速率最快，且在静息和去极化状态下摄取速率均迅速下降。5. 在15秒时，SHR和WKY之间存在显著差异，而WKY和SD之间无显著差异。6. 结果表明，SHR脑突触体中Ca2+通道特性可能发生了重要改变。